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| | <StructureSection load='1c8o' size='340' side='right'caption='[[1c8o]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='1c8o' size='340' side='right'caption='[[1c8o]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1c8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpx Cowpx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1c8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8O FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8o OCA], [https://pdbe.org/1c8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8o RCSB], [https://www.ebi.ac.uk/pdbsum/1c8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8o ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8o OCA], [https://pdbe.org/1c8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8o RCSB], [https://www.ebi.ac.uk/pdbsum/1c8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB]] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
| + | [https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cowpx]] | + | [[Category: Cowpox virus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gettins, P G.W]] | + | [[Category: Gettins PGW]] |
| - | [[Category: Simonovic, M]] | + | [[Category: Simonovic M]] |
| - | [[Category: Volz, K]] | + | [[Category: Volz K]] |
| - | [[Category: Serpin fold]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
SPI2_CWPXB Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition.,Simonovic M, Gettins PGW, Volz K Protein Sci. 2000 Aug;9(8):1423-7. PMID:10975564[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ray CA, Black RA, Kronheim SR, Greenstreet TA, Sleath PR, Salvesen GS, Pickup DJ. Viral inhibition of inflammation: cowpox virus encodes an inhibitor of the interleukin-1 beta converting enzyme. Cell. 1992 May 15;69(4):597-604. PMID:1339309
- ↑ Komiyama T, Ray CA, Pickup DJ, Howard AD, Thornberry NA, Peterson EP, Salvesen G. Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. An example of cross-class inhibition. J Biol Chem. 1994 Jul 29;269(30):19331-7. PMID:8034697
- ↑ Simonovic M, Gettins PGW, Volz K. Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition. Protein Sci. 2000 Aug;9(8):1423-7. PMID:10975564
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