1eq9
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1eq9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solenopsis_invicta Solenopsis invicta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQ9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1eq9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solenopsis_invicta Solenopsis invicta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQ9 FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eq9 OCA], [https://pdbe.org/1eq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eq9 RCSB], [https://www.ebi.ac.uk/pdbsum/1eq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eq9 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eq9 OCA], [https://pdbe.org/1eq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eq9 RCSB], [https://www.ebi.ac.uk/pdbsum/1eq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eq9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CTR1_SOLIN CTR1_SOLIN] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eq9 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eq9 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The South American imported fire ant (Solenopsis invicta), without natural enemies in the United States, widely infests the southern United States, causing more than a half billion dollars in health and agriculture-related damage annually in Texas alone. Fire ants are resistant to most insecticides, so control will require a more fundamental understanding of their biochemistry and metabolism leading to the design of selective, ecologically safe insecticides. The 4th instar larvae play a crucial role in the nutrition of the colony by secreting proteinases (especially chymotrypsin) which digest food products for the entire colony. The first structure of an ant proteolytic enzyme, fire ant chymotrypsin, was determined to atomic resolution (1.7 A). A structural comparison of the ant and mammalian structures confirms the "universality" of the serine proteinase motif and reveals a difference at residues 147-148, which are proteolytically removed in the bovine enzyme but are firmly intact in the ant chymotrypsin, suggesting a different activation mechanism for the latter. Likewise, the absence of the covalently attached propeptide domain (1-15) further suggests an uncharacteristic activation mechanism. The presence of Gly189 in the S1 site is an atypical feature of this chymotrypsin and is comparable only to human leukocyte elastase, hornet chymotrypsin and fiddler crab collagenase. Binding studies confirm the chymotrypsin nature of this novel enzyme. | ||
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| - | The structure of an insect chymotrypsin.,Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF J Mol Biol. 2000 May 19;298(5):895-901. PMID:10801356<ref>PMID:10801356</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1eq9" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]] | *[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Chymotrypsin]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Solenopsis invicta]] | [[Category: Solenopsis invicta]] | ||
| - | [[Category: Botos | + | [[Category: Botos I]] |
| - | [[Category: Meyer | + | [[Category: Meyer E]] |
| - | [[Category: Meyer | + | [[Category: Meyer EF]] |
| - | [[Category: Nguyen | + | [[Category: Nguyen MH]] |
| - | [[Category: Swanson | + | [[Category: Swanson SM]] |
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Revision as of 10:05, 20 March 2024
CRYSTAL STRUCTURE OF FIRE ANT CHYMOTRYPSIN COMPLEXED TO PMSF
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