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1ewx

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[[Image:1ewx.gif|left|200px]]
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{{STRUCTURE_1ewx| PDB=1ewx | SCENE= }}
{{STRUCTURE_1ewx| PDB=1ewx | SCENE= }}
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'''Crystal structure of native tryparedoxin I from Crithidia fasciculata'''
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===Crystal structure of native tryparedoxin I from Crithidia fasciculata===
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==Overview==
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Tryparedoxins (TXNs) catalyse the reduction of peroxiredoxin-type peroxidases by the bis-glutathionyl derivative of spermidine, trypanothione, and are relevant to hydroperoxide detoxification and virulence of trypanosomes. The 3D-structures of the following tryparedoxins are presented: authentic tryparedoxin1 of Crithidia fasciculata, CfTXN1; the his-tagged recombinant protein, CfTXN1H6; reduced and oxidised CfTXN2, and an alternative substrate derivative of the mutein CfTXN2H6-Cys44Ser. Cys41 (Cys40 in TXN1) of the active site motif 40-WCPPCR-45 proved to be the only solvent-exposed redox active residue in CfTXN2. In reduced TXNs, its nucleophilicity is increased by a network of hydrogen bonds. In oxidised TXNs it can be attacked by the thiol of the 1N-glutathionyl residue of trypanothione, as evidenced by the structure of 1N-glutathionylspermidine-derivatised CfTXN2H6-Cys44Ser. Modelling suggests Arg45 (44), Glu73 (72), the Ile110 (109) cis-Pro111 (110)-bond and Arg129 (128) to be involved in the binding of trypanothione to CfTXN2 (CfTXN1). The model of TXN-substrate interaction is consistent with functional characteristics of known and newly designed muteins (CfTXN2H6-Arg129Asp and Glu73Arg) and the 1N-glutathionyl-spermidine binding in the CfTXN2H6-Cys44Ser structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 11347894 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11347894}}
==About this Structure==
==About this Structure==
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[[Category: Singh, M.]]
[[Category: Singh, M.]]
[[Category: Electron transport]]
[[Category: Electron transport]]
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Revision as of 23:08, 30 June 2008

Image:1ewx.png

Template:STRUCTURE 1ewx

Crystal structure of native tryparedoxin I from Crithidia fasciculata

Template:ABSTRACT PUBMED 11347894

About this Structure

1EWX is a Single protein structure of sequence from Crithidia fasciculata. Full crystallographic information is available from OCA.

Reference

Structures of tryparedoxins revealing interaction with trypanothione., Hofmann B, Budde H, Bruns K, Guerrero SA, Kalisz HM, Menge U, Montemartini M, Nogoceke E, Steinert P, Wissing JB, Flohe L, Hecht HJ, Biol Chem. 2001 Mar;382(3):459-71. PMID:11347894

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