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1exd

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{{STRUCTURE_1exd| PDB=1exd | SCENE= }}
{{STRUCTURE_1exd| PDB=1exd | SCENE= }}
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'''CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE'''
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===CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE===
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==Overview==
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Guided by an in vitro selection experiment designed to obtain tight binding aptamers of Escherichia coli glutamine specific tRNA (tRNAGln) for glutaminyl-tRNA synthetase (GlnRS), we have engineered a tRNA mutant in which the five-nucleotide variable loop sequence 5'-44CAUUC48-3' is replaced by 5'-44AGGU48-3'. This mutant tRNA binds to GlnRS with 30-fold improved affinity compared to the wild type. The 2.7 A cocrystal structure of the RNA aptamer-GlnRS complex reveals major rearrangements in the central tertiary core of the tRNA, while maintaining an RNA-protein interface identical to the wild type. The repacked RNA core features a novel hydrogen bonding arrangement of the trans Levitt pair G15-U48, a new sulfate binding pocket in the major groove, and increased hydrophobic stacking interactions among the bases. These data suggest that enhanced protein binding to a mutant globular RNA can arise from stabilization of RNA tertiary interactions rather than optimization of RNA-protein contacts.
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(as it appears on PubMed at http://www.pubmed.gov), where 10881199 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10881199}}
==About this Structure==
==About this Structure==
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[[Category: Trna aptamer]]
[[Category: Trna aptamer]]
[[Category: Trna-protein complex]]
[[Category: Trna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:09:59 2008''

Revision as of 23:10, 30 June 2008

Image:1exd.png

Template:STRUCTURE 1exd

CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE

Template:ABSTRACT PUBMED 10881199

About this Structure

1EXD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Tertiary core rearrangements in a tight binding transfer RNA aptamer., Bullock TL, Sherlin LD, Perona JJ, Nat Struct Biol. 2000 Jun;7(6):497-504. PMID:10881199

Page seeded by OCA on Tue Jul 1 02:09:59 2008

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