7d5k
From Proteopedia
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==CryoEM structure of cotton cellulose synthase isoform 7== | ==CryoEM structure of cotton cellulose synthase isoform 7== | ||
| - | <StructureSection load='7d5k' size='340' side='right'caption='[[7d5k]]' scene=''> | + | <StructureSection load='7d5k' size='340' side='right'caption='[[7d5k]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D5K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7d5k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/American_cotton American cotton]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D5K FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d5k OCA], [https://pdbe.org/7d5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d5k RCSB], [https://www.ebi.ac.uk/pdbsum/7d5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d5k ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CesA7, LOC107953681 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3635 American cotton])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulose_synthase_(UDP-forming) Cellulose synthase (UDP-forming)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.12 2.4.1.12] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d5k OCA], [https://pdbe.org/7d5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d5k RCSB], [https://www.ebi.ac.uk/pdbsum/7d5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d5k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellulose is one of the most abundant organic polymers in nature. It contains multiple beta-1,4-glucan chains synthesized by cellulose synthases (CesAs) on the plasma membrane of higher plants. CesA subunits assemble into a pseudo-sixfold symmetric cellulose synthase complex (CSC), known as a 'rosette complex'. The structure of CesA remains enigmatic. Here, we report the cryo-EM structure of the homotrimeric CesA7 from Gossypium hirsutum at 3.5-angstrom resolution. The GhCesA7 homotrimer shows a C3 symmetrical assembly. Each protomer contains seven transmembrane helices (TMs) which form a channel potentially facilitating the release of newly synthesized glucans. The cytoplasmic glycosyltransferase domain (GT domain) of GhCesA7 protrudes from the membrane, and its catalytic pocket is directed towards the TM pore. The homotrimer GhCesA7 is stabilized by the transmembrane helix 7 (TM7) and the plant-conserved region (PCR) domains. It represents the building block of CSCs and facilitates microfibril formation. This structure provides insight into how eukaryotic cellulose synthase assembles and provides a mechanistic basis for the improvement of cotton fibre quality in the future. | ||
| + | |||
| + | Structural insights into homotrimeric assembly of cellulose synthase CesA7 from Gossypium hirsutum.,Zhang X, Xue Y, Guan Z, Zhou C, Nie Y, Men S, Wang Q, Shen C, Zhang D, Jin S, Tu L, Yin P, Zhang X Plant Biotechnol J. 2021 Feb 26. doi: 10.1111/pbi.13571. PMID:33638282<ref>PMID:33638282</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7d5k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: American cotton]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Guan | + | [[Category: Guan, Z Y]] |
| - | [[Category: Xue Y]] | + | [[Category: Xue, Y]] |
| - | [[Category: Yin P]] | + | [[Category: Yin, P]] |
| - | [[Category: Zhang | + | [[Category: Zhang, X L]] |
| + | [[Category: Cellulose synthase]] | ||
| + | [[Category: Cotton]] | ||
| + | [[Category: Membrane protein]] | ||
Revision as of 05:52, 6 October 2021
CryoEM structure of cotton cellulose synthase isoform 7
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