7m99
From Proteopedia
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| - | ==== | + | ==ATPgS bound TnsC filament from ShCAST system== |
| - | <StructureSection load='7m99' size='340' side='right'caption='[[7m99]]' scene=''> | + | <StructureSection load='7m99' size='340' side='right'caption='[[7m99]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7m99]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M99 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m99 OCA], [https://pdbe.org/7m99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m99 RCSB], [https://www.ebi.ac.uk/pdbsum/7m99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m99 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m99 OCA], [https://pdbe.org/7m99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m99 RCSB], [https://www.ebi.ac.uk/pdbsum/7m99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m99 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. We used cryo-electron microscopy (cryo-EM) to define the mechanism that underlies this process by characterizing the transposition regulator, TnsC, from a type V-K CRISPR-transposase system. In this scenario, polymerization of adenosine triphosphate-bound TnsC helical filaments could explain how polarity information is passed to the transposase. TniQ caps the TnsC filament, representing a universal mechanism for target information transfer in Tn7/Tn7-like elements. Transposase-driven disassembly establishes delivery of the element only to unused protospacers. Finally, TnsC transitions to define the fixed point of insertion, as revealed by structures with the transition state mimic ADP*AlF(3) These mechanistic findings provide the underpinnings for engineering CRISPR-associated transposition systems for research and therapeutic applications. | ||
| + | |||
| + | Structural basis for target site selection in RNA-guided DNA transposition systems.,Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH Science. 2021 Aug 13;373(6556):768-774. doi: 10.1126/science.abi8976. Epub 2021 , Jul 15. PMID:34385391<ref>PMID:34385391</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7m99" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Transposase 3D structures|Transposase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Scytonema hofmannii]] |
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Kellogg EH]] | ||
| + | [[Category: Mehrotra E]] | ||
| + | [[Category: Park J]] | ||
| + | [[Category: Tsai AWL]] | ||
Revision as of 06:53, 25 January 2023
ATPgS bound TnsC filament from ShCAST system
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