6zni

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:41, 1 May 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='6zni' size='340' side='right'caption='[[6zni]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<StructureSection load='6zni' size='340' side='right'caption='[[6zni]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6zni]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Shifm Shifm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNI FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6zni]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_5a_str._M90T Shigella flexneri 5a str. M90T]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNI FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mxiH, CP0137 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1086030 SHIFM])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zni OCA], [https://pdbe.org/6zni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zni RCSB], [https://www.ebi.ac.uk/pdbsum/6zni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zni ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zni OCA], [https://pdbe.org/6zni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zni RCSB], [https://www.ebi.ac.uk/pdbsum/6zni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zni ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL]] Necessary for the secretion of IPA invasins.
+
[https://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL] Necessary for the secretion of IPA invasins.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.
+
-
 
+
-
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.,Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC Biochem Biophys Rep. 2021 Jun 27;27:101039. doi: 10.1016/j.bbrep.2021.101039., eCollection 2021 Sep. PMID:34258394<ref>PMID:34258394</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6zni" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Shifm]]
+
[[Category: Shigella flexneri 5a str. M90T]]
-
[[Category: Kolbe, M]]
+
[[Category: Kolbe M]]
-
[[Category: Kotov, V]]
+
[[Category: Kotov V]]
-
[[Category: Lunelli, M]]
+
[[Category: Lunelli M]]
-
[[Category: Marlovits, T C]]
+
[[Category: Marlovits TC]]
-
[[Category: Helical reconstruction]]
+
-
[[Category: Needle filament]]
+
-
[[Category: Protein transport]]
+
-
[[Category: Shigella]]
+
-
[[Category: Type 3 secretion system]]
+

Current revision

Structure of the Shigella MxiH needle filament attached to the basal body

PDB ID 6zni

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6zni"
Personal tools