1eyn

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{{STRUCTURE_1eyn| PDB=1eyn | SCENE= }}
{{STRUCTURE_1eyn| PDB=1eyn | SCENE= }}
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'''STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS'''
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===STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS===
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==Overview==
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The extrinsic fluorescence dye 8-anilino-1-naphthalene sulfonate (ANS) is widely used for probing conformational changes in proteins, yet no detailed structure of ANS bound to any protein has been reported so far. ANS has been successfully used to monitor the induced-fit mechanism of MurA [UDPGlcNAc enolpyruvyltransferase (EC )], an essential enzyme for bacterial cell wall biosynthesis. We have solved the crystal structure of the ANS small middle dotMurA complex at 1.7-A resolution. ANS binds at an originally solvent-exposed region near Pro-112 and induces a major restructuring of the loop Pro-112-Pro-121, such that a specific binding site emerges. The fluorescence probe is sandwiched between the strictly conserved residues Arg-91, Pro-112, and Gly-113. Substrate binding to MurA is accompanied by large movements especially of the loop and Arg-91, which explains why ANS is an excellent sensor of conformational changes during catalysis of this pharmaceutically important enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 10823915 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10823915}}
==About this Structure==
==About this Structure==
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[[Category: Inside-out alpha-beta barrel]]
[[Category: Inside-out alpha-beta barrel]]
[[Category: L-isoaspartate in position 67]]
[[Category: L-isoaspartate in position 67]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:40:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:13:19 2008''

Revision as of 23:13, 30 June 2008

Image:1eyn.png

Template:STRUCTURE 1eyn

STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS

Template:ABSTRACT PUBMED 10823915

About this Structure

1EYN is a Single protein structure of sequence from Enterobacter cloacae. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA., Schonbrunn E, Eschenburg S, Luger K, Kabsch W, Amrhein N, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6345-9. PMID:10823915

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