6zvy
From Proteopedia
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==X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174R LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE== | ==X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174R LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE== | ||
| - | <StructureSection load='6zvy' size='340' side='right'caption='[[6zvy]]' scene=''> | + | <StructureSection load='6zvy' size='340' side='right'caption='[[6zvy]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZVY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zvy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhoso Rhoso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZVY FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zvy OCA], [https://pdbe.org/6zvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zvy RCSB], [https://www.ebi.ac.uk/pdbsum/6zvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zvy ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OEH:[9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium'>OEH</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6zvu|6zvu]], [[6zvv|6zvv]], [[6zvx|6zvx]], [[6zvw|6zvw]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dhaA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1831 RHOSO])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zvy OCA], [https://pdbe.org/6zvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zvy RCSB], [https://www.ebi.ac.uk/pdbsum/6zvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zvy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications. | ||
| + | |||
| + | Engineered HaloTag variants for fluorescence lifetime multiplexing.,Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K Nat Methods. 2022 Jan;19(1):65-70. doi: 10.1038/s41592-021-01341-x. Epub 2021 Dec, 16. PMID:34916672<ref>PMID:34916672</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zvy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Haloalkane dehalogenase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Frei M]] | + | [[Category: Rhoso]] |
| - | [[Category: Hiblot J]] | + | [[Category: Frei, M]] |
| - | [[Category: Johnsson K]] | + | [[Category: Hiblot, J]] |
| - | [[Category: Tarnawski M]] | + | [[Category: Johnsson, K]] |
| + | [[Category: Tarnawski, M]] | ||
| + | [[Category: Fluorophore]] | ||
| + | [[Category: Halo]] | ||
| + | [[Category: Halotag7]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Self-labeling protein]] | ||
| + | [[Category: Tag]] | ||
| + | [[Category: Tetramethylrhodamine]] | ||
Revision as of 10:47, 16 February 2022
X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174R LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE
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