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1ezn

From Proteopedia

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[[Image:1ezn.gif|left|200px]]
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{{STRUCTURE_1ezn| PDB=1ezn | SCENE= }}
{{STRUCTURE_1ezn| PDB=1ezn | SCENE= }}
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'''SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION'''
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===SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION===
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==Overview==
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The solution structure of a DNA three-way junction (3H) containing two unpaired thymidine bases at the branch site (3HT2), was determined by NMR. Arms A and B of the 3HT2 form a quasi-continuous stacked helix, which is underwound at the junction and has an increased helical rise. The unstacked arm C forms an acute angle of approximately 55 degrees with the unique arm A. The stacking of the unpaired thymidine bases on arm C resembles the folding of hairpin loops. From this data, combined with the reported stacking behavior of 23 other 3HS2 s, two rules are derived that together correctly reproduce their stacking preference. These rules predict, from the sequence of any 3HS2, its stacking preference. The structure also suggests a plausible mechanism for structure-specific recognition of branched nucleic acids by proteins.
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The line below this paragraph, {{ABSTRACT_PUBMED_11090280}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11090280 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11090280}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZN OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZN OCA].
==Reference==
==Reference==
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[[Category: Holliday junction]]
[[Category: Holliday junction]]
[[Category: Three-way junction]]
[[Category: Three-way junction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:42:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:15:44 2008''

Revision as of 23:15, 30 June 2008

Image:1ezn.png

Template:STRUCTURE 1ezn

SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION

Template:ABSTRACT PUBMED 11090280

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection., van Buuren BN, Overmars FJ, Ippel JH, Altona C, Wijmenga SS, J Mol Biol. 2000 Dec 1;304(3):371-83. PMID:11090280

Page seeded by OCA on Tue Jul 1 02:15:44 2008

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