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1f0o

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[[Image:1f0o.gif|left|200px]]
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{{STRUCTURE_1f0o| PDB=1f0o | SCENE= }}
{{STRUCTURE_1f0o| PDB=1f0o | SCENE= }}
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'''PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE'''
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===PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE===
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==Overview==
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Restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. To explore this, we have characterized the interaction of endonuclease PvuII with the catalytically incompetent cation Ca(2+). The structure of a glutaraldehyde-crosslinked crystal of the endonuclease PvuII-DNA complex, determined in the presence of Ca(2+) at a pH of approximately 6.5, supports a two-metal mechanism of DNA cleavage by PvuII. The first Ca(2+) position matches that found in all structurally examined endonucleases, while the second position is similar to that of EcoRV but is distinct from that of BamHI and BglI. The location of the second metal in PvuII, unlike that in BamHI/BglI, permits no direct interaction between the second metal and the O3' oxygen leaving group. However, the interactions between the DNA scissile phosphate and the metals, the first metal and the attacking water, and the attacking water and DNA are the same in PvuII as they are in the two-metal models of BamHI and BglI, but are distinct from the proposed three-metal or the two-metal models of EcoRV.
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The line below this paragraph, {{ABSTRACT_PUBMED_10903853}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10903853 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10903853}}
==About this Structure==
==About this Structure==
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[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Restriction enzyme]]
[[Category: Restriction enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:45:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:18:22 2008''

Revision as of 23:18, 30 June 2008

Image:1f0o.png

Template:STRUCTURE 1f0o

PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE

Template:ABSTRACT PUBMED 10903853

About this Structure

1F0O is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

Reference

PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:10903853

Page seeded by OCA on Tue Jul 1 02:18:22 2008

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