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| | <StructureSection load='1jqg' size='340' side='right'caption='[[1jqg]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='1jqg' size='340' side='right'caption='[[1jqg]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jqg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/American_bollworm American bollworm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jqg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera Helicoverpa armigera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqg OCA], [https://pdbe.org/1jqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqg RCSB], [https://www.ebi.ac.uk/pdbsum/1jqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqg ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqg OCA], [https://pdbe.org/1jqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqg RCSB], [https://www.ebi.ac.uk/pdbsum/1jqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O97389_HELAM O97389_HELAM] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: American bollworm]] | + | [[Category: Helicoverpa armigera]] |
| - | [[Category: Carboxypeptidase A]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aviles, F X]] | + | [[Category: Aviles FX]] |
| - | [[Category: Bayes, A]] | + | [[Category: Bayes A]] |
| - | [[Category: Bode, W]] | + | [[Category: Bode W]] |
| - | [[Category: Bown, D P]] | + | [[Category: Bown DP]] |
| - | [[Category: Estebanez-Perpina, E]] | + | [[Category: Estebanez-Perpina E]] |
| - | [[Category: Gatehouse, J A]] | + | [[Category: Gatehouse JA]] |
| - | [[Category: Huber, R]] | + | [[Category: Huber R]] |
| - | [[Category: Jongsma, M A]] | + | [[Category: Jongsma MA]] |
| - | [[Category: Reverter, D]] | + | [[Category: Reverter D]] |
| - | [[Category: Vendrell, J]] | + | [[Category: Vendrell J]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pro-protein]]
| + | |
| Structural highlights
Function
O97389_HELAM
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The cotton bollworm Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is one of the most serious insect pests in Australia, India and China. The larva causes substantial economical losses to legume, fibre, cereal oilseed and vegetable crops. This pest has proven to be difficult to control by conventional means, mainly due to the development of pesticide resistance. We present here the 2.5 A crystal structure from the novel procarboxypeptidase (PCPAHa) found in the gut extracts from H. armigera larvae, the first one reported for an insect. This metalloprotease is synthesized as a zymogen of 46.6 kDa which, upon in vitro activation with Lys-C endoproteinase, yields a pro-segment of 91 residues and an active carboxypeptidase moiety of 318 residues. Both regions show a three-dimensional structure quite similar to the corresponding structures in mammalian digestive carboxypeptidases, the most relevant structural differences being located in the loops between conserved secondary structure elements, including the primary activation site. This activation site contains the motif (Ala)(5)Lys at the C terminus of the helix connecting the pro- and the carboxypeptidase domains. A remarkable feature of PCPAHa is the occurrence of the same (Ala)(6)Lys near the C terminus of the active enzyme. The presence of Ser255 in PCPAHa instead of Ile and Asp found in the pancreatic A and B forms, respectively, enlarges the S1' specificity pocket and influences the substrate preferences of the enzyme. The C-terminal tail of the leech carboxypeptidase inhibitor has been modelled into the PCPAHa active site to explore the substrate preferences and the enzymatic mechanism of this enzyme.
Crystal structure of a novel mid-gut procarboxypeptidase from the cotton pest Helicoverpa armigera.,Estebanez-Perpina E, Bayes A, Vendrell J, Jongsma MA, Bown DP, Gatehouse JA, Huber R, Bode W, Aviles FX, Reverter D J Mol Biol. 2001 Oct 26;313(3):629-38. PMID:11676544[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Estebanez-Perpina E, Bayes A, Vendrell J, Jongsma MA, Bown DP, Gatehouse JA, Huber R, Bode W, Aviles FX, Reverter D. Crystal structure of a novel mid-gut procarboxypeptidase from the cotton pest Helicoverpa armigera. J Mol Biol. 2001 Oct 26;313(3):629-38. PMID:11676544 doi:10.1006/jmbi.2001.5076
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