Transcription-repair coupling factor
From Proteopedia
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| - | + | The bacterial transcription-repair coupling factor TRCF, also called Mfd translocase, is a DNA repair protein. It has a role in transcription-coupled repair, a subpathway of nucleotide excision repair (NER). Mfd recognizes stalled RNA polymerase and either restarts transcription or removes the stalled polymerase and recruits the NER proteins UvrA and UvrB. | |
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== Function == | == Function == | ||
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Size exclusion chromatogaphy indicates that the protein <scene name='2eyq/Biolunit/1'>exists as a monomer in solution.</scene> | Size exclusion chromatogaphy indicates that the protein <scene name='2eyq/Biolunit/1'>exists as a monomer in solution.</scene> | ||
== Structural highlights == | == Structural highlights == | ||
| + | <StructureSection load='' size='350' side='right' scene='2eyq/Domainscolorsflabe/3' caption=''> | ||
<scene name='2eyq/Figonea/2'>The monomer colored as</scene> an N→C rainbow similar to [http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6WSN-4J79KGF-J&_image=fig1&_ba=1&_user=10&_rdoc=1&_fmt=full&_orig=search&_cdi=7051&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a4326e4023d32f5de977da04b9a9fdf6 Figure 1A] of [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4J79KGF-J&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cd0b2f15037bec97859866e118038962 the article describing] the structure. {{Link Toggle FancyCartoonHighQualityView}}. | <scene name='2eyq/Figonea/2'>The monomer colored as</scene> an N→C rainbow similar to [http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6WSN-4J79KGF-J&_image=fig1&_ba=1&_user=10&_rdoc=1&_fmt=full&_orig=search&_cdi=7051&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a4326e4023d32f5de977da04b9a9fdf6 Figure 1A] of [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4J79KGF-J&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cd0b2f15037bec97859866e118038962 the article describing] the structure. {{Link Toggle FancyCartoonHighQualityView}}. | ||
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<scene name='2eyq/Figure3c/3'>TRG motif and motif VI</scene> indicated similar to [http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6WSN-4J79KGF-J&_image=fig3&_ba=3&_user=10&_rdoc=1&_fmt=full&_orig=search&_cdi=7051&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=580f83ffa975d90b5c9d9dc0eb930d8c Figure 3C] of [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4J79KGF-J&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cd0b2f15037bec97859866e118038962 the article describing] the structure. | <scene name='2eyq/Figure3c/3'>TRG motif and motif VI</scene> indicated similar to [http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6WSN-4J79KGF-J&_image=fig3&_ba=3&_user=10&_rdoc=1&_fmt=full&_orig=search&_cdi=7051&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=580f83ffa975d90b5c9d9dc0eb930d8c Figure 3C] of [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4J79KGF-J&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cd0b2f15037bec97859866e118038962 the article describing] the structure. | ||
| + | </StructureSection> | ||
== 3D Structures of Transcription-repair coupling factor == | == 3D Structures of Transcription-repair coupling factor == | ||
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | [[2eyq | + | [[2eyq]] – EcTRCF – ''Escherichia coli''<br /> |
| + | [[6X2N]], [[6X2F]], [[6X26]], [[6X50]], [[6X43]], [[6X4W]], [[6X4Y]] - EcTRCF, RNA polymerase, RNA, DNA (Cryo EM) /> | ||
[[3hjh]] – EcTRCF residues 1-470<br /> | [[3hjh]] – EcTRCF residues 1-470<br /> | ||
| + | [[2b2n]] - EcTRCF residues 1-333<br /> | ||
[[6yhz]] - EcTRCF residues 472-547 – NMR<br /> | [[6yhz]] - EcTRCF residues 472-547 – NMR<br /> | ||
[[4dfc]] – EcTRCF D2 domain 127-213 + UvrABC system protein A<br /> | [[4dfc]] – EcTRCF D2 domain 127-213 + UvrABC system protein A<br /> | ||
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[[6acx]] – MsTRCF + ADP<br /> | [[6acx]] – MsTRCF + ADP<br /> | ||
| - | </StructureSection> | ||
==Reference== | ==Reference== | ||
Revision as of 00:23, 15 August 2021
The bacterial transcription-repair coupling factor TRCF, also called Mfd translocase, is a DNA repair protein. It has a role in transcription-coupled repair, a subpathway of nucleotide excision repair (NER). Mfd recognizes stalled RNA polymerase and either restarts transcription or removes the stalled polymerase and recruits the NER proteins UvrA and UvrB.
Contents |
Function
Transcription-repair coupling factor (TRCF) or Mfd enables the coupling of these processes in bacteria and humans. The TRCF has ATPase activity.
Size exclusion chromatogaphy indicates that the protein
Structural highlights
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3D Structures of Transcription-repair coupling factor
Updated on 15-August-2021
2eyq – EcTRCF – Escherichia coli
6X2N, 6X2F, 6X26, 6X50, 6X43, 6X4W, 6X4Y - EcTRCF, RNA polymerase, RNA, DNA (Cryo EM) />
3hjh – EcTRCF residues 1-470
2b2n - EcTRCF residues 1-333
6yhz - EcTRCF residues 472-547 – NMR
4dfc – EcTRCF D2 domain 127-213 + UvrABC system protein A
6xeo – EcTRCF + DNA – Cryo EM
3mlq – TtTRCF RNA polymerase interacting domain + DNA-directed RNA polymerase subunit β - Thermus thermophilus
6m6a – TtTRCF + RNA polymerase – Cryo EM
6m6b – TtTRCF + RNA polymerase + ATP-γ-S – Cryo EM
2qsr – TRCF C terminal – Streptococcus pneumoni
6ac6, 6aca, 6ac8 – MsTRCF – Mycobacterium smegmatis
6acx – MsTRCF + ADP
Reference
- Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA. Structural basis for bacterial transcription-coupled DNA repair. Cell. 2006 Feb 10;124(3):507-20. PMID:16469698 doi:10.1016/j.cell.2005.11.045
Created with the participation of Wayne Decatur
Proteopedia Page Contributors and Editors (what is this?)
Karsten Theis, Michal Harel, Alexander Berchansky, Wayne Decatur
