1hiy
From Proteopedia
(New page: 200px<br /> <applet load="1hiy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hiy, resolution 2.6Å" /> '''BINDING OF NUCLEOTID...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1HIY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]] with 3AN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HIY OCA]]. | + | 1HIY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]] with 3AN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6]]. Structure known Active Sites: AC1, AC2 and AC3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HIY OCA]]. |
==Reference== | ==Reference== | ||
Binding of nucleotides to nucleoside diphosphate kinase: a calorimetric study., Cervoni L, Lascu I, Xu Y, Gonin P, Morr M, Merouani M, Janin J, Giartosio A, Biochemistry. 2001 Apr 17;40(15):4583-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11294625 11294625] | Binding of nucleotides to nucleoside diphosphate kinase: a calorimetric study., Cervoni L, Lascu I, Xu Y, Gonin P, Morr M, Merouani M, Janin J, Giartosio A, Biochemistry. 2001 Apr 17;40(15):4583-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11294625 11294625] | ||
[[Category: Dictyostelium discoideum]] | [[Category: Dictyostelium discoideum]] | ||
| + | [[Category: Nucleoside-diphosphate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cervoni, L.]] | [[Category: Cervoni, L.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:31:09 2007'' |
Revision as of 09:26, 30 October 2007
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BINDING OF NUCLEOTIDES TO NDP KINASE
Overview
The source of affinity for substrates of human nucleoside diphosphate, (NDP) kinases is particularly important in that its knowledge could be, used to design more effective antiviral nucleoside drugs (e.g., AZT). We, carried out a microcalorimetric study of the binding of enzymes from two, organisms to various nucleotides. Isothermal titration calorimetry has, been used to characterize the binding in terms of Delta G degrees, Delta H, degrees and Delta S degrees. Thermodynamic parameters of the interaction, of ADP with the hexameric NDP kinase from Dictyostelium discoideum and, with the tetrameric enzyme from Myxococcus xanthus, at 20 degrees C, were, similar and, in both cases, binding was enthalpy-driven. The interactions, of ADP, 2'deoxyADP, GDP, and IDP with the eukaryotic enzyme ... [(full description)]
About this Structure
1HIY is a [Single protein] structure of sequence from [Dictyostelium discoideum] with 3AN as [ligand]. Active as [Nucleoside-diphosphate kinase], with EC number [2.7.4.6]. Structure known Active Sites: AC1, AC2 and AC3. Full crystallographic information is available from [OCA].
Reference
Binding of nucleotides to nucleoside diphosphate kinase: a calorimetric study., Cervoni L, Lascu I, Xu Y, Gonin P, Morr M, Merouani M, Janin J, Giartosio A, Biochemistry. 2001 Apr 17;40(15):4583-9. PMID:11294625
Page seeded by OCA on Tue Oct 30 11:31:09 2007
