7p13
From Proteopedia
(Difference between revisions)
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==2.29 A Mycobacterium tuberculosis EspB.== | ==2.29 A Mycobacterium tuberculosis EspB.== | ||
- | <StructureSection load='7p13' size='340' side='right'caption='[[7p13]]' scene=''> | + | <StructureSection load='7p13' size='340' side='right'caption='[[7p13]], [[Resolution|resolution]] 2.29Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P13 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p13]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P13 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p13 OCA], [https://pdbe.org/7p13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p13 RCSB], [https://www.ebi.ac.uk/pdbsum/7p13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p13 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p13 OCA], [https://pdbe.org/7p13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p13 RCSB], [https://www.ebi.ac.uk/pdbsum/7p13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p13 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | ESX-1 is a major virulence factor of Mycobacterium tuberculosis, a secretion machinery directly involved in the survival of the microorganism from the immune system defence. It disrupts the phagosome membrane of the host cell through a contact-dependent mechanism. Recently, the structure of the inner-membrane core complex of the homologous ESX-3 and ESX-5 was resolved; however, the elements involved in the secretion through the outer membrane or those acting on the host cell membrane are unknown. Protein substrates might form this missing element. Here, we describe the oligomerisation process of the ESX-1 substrate EspB, which occurs upon cleavage of its C-terminal region and is favoured by an acidic environment. Cryo-electron microscopy data shows that quaternary structure of EspB is conserved across slow growing species, but not in the fast growing M. smegmatis. EspB assembles into a channel with dimensions and characteristics suitable for the transit of ESX-1 substrates, as shown by the presence of another EspB trapped within. Our results provide insight into the structure and assembly of EspB, and suggests a possible function as a structural element of ESX-1. | ||
+ | |||
+ | Priming mycobacterial ESX-secreted protein B to form a channel-like structure.,Gijsbers A, Vinciauskaite V, Siroy A, Gao Y, Tria G, Mathew A, Sanchez-Puig N, Lopez-Iglesias C, Peters PJ, Ravelli RBG Curr Res Struct Biol. 2021 Jun 30;3:153-164. doi: 10.1016/j.crstbi.2021.06.001., eCollection 2021. PMID:34337436<ref>PMID:34337436</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7p13" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
- | [[Category: Gao Y]] | + | [[Category: Myctu]] |
- | [[Category: Gijsbers A]] | + | [[Category: Gao, Y]] |
- | [[Category: Lopez-Iglesias C]] | + | [[Category: Gijsbers, A]] |
- | [[Category: Mathew A]] | + | [[Category: Lopez-Iglesias, C]] |
- | [[Category: Peters | + | [[Category: Mathew, A]] |
- | [[Category: Ravelli | + | [[Category: Peters, P J]] |
- | [[Category: Sanchez-Puig N]] | + | [[Category: Ravelli, R B.G]] |
- | [[Category: Siroy A]] | + | [[Category: Sanchez-Puig, N]] |
- | [[Category: Tria G]] | + | [[Category: Siroy, A]] |
- | [[Category: Vinciauskaite V]] | + | [[Category: Tria, G]] |
- | [[Category: Zhang Y]] | + | [[Category: Vinciauskaite, V]] |
+ | [[Category: Zhang, Y]] | ||
+ | [[Category: Cryo-em]] | ||
+ | [[Category: Espb]] | ||
+ | [[Category: Esx-1]] | ||
+ | [[Category: Preferential orientation]] | ||
+ | [[Category: Protein transport]] |
Revision as of 12:58, 13 October 2021
2.29 A Mycobacterium tuberculosis EspB.
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Categories: Large Structures | Myctu | Gao, Y | Gijsbers, A | Lopez-Iglesias, C | Mathew, A | Peters, P J | Ravelli, R B.G | Sanchez-Puig, N | Siroy, A | Tria, G | Vinciauskaite, V | Zhang, Y | Cryo-em | Espb | Esx-1 | Preferential orientation | Protein transport