7a3w
From Proteopedia
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==Structure of Imine Reductase from Pseudomonas sp.== | ==Structure of Imine Reductase from Pseudomonas sp.== | ||
| - | <StructureSection load='7a3w' size='340' side='right'caption='[[7a3w]]' scene=''> | + | <StructureSection load='7a3w' size='340' side='right'caption='[[7a3w]], [[Resolution|resolution]] 1.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7a3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3W FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3w OCA], [https://pdbe.org/7a3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3w RCSB], [https://www.ebi.ac.uk/pdbsum/7a3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3w ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3w OCA], [https://pdbe.org/7a3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3w RCSB], [https://www.ebi.ac.uk/pdbsum/7a3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chiral amine diastereomers are ubiquitous in pharmaceuticals and agrochemicals(1), yet their preparation often relies on low-efficiency multi-step synthesis(2). These valuable compounds must be manufactured asymmetrically, as their biochemical properties can differ based on the chirality of the molecule. Herein we characterize a multifunctional biocatalyst for amine synthesis, which operates using a mechanism that is, to our knowledge, previously unreported. This enzyme (EneIRED), identified within a metagenomic imine reductase (IRED) collection(3) and originating from an unclassified Pseudomonas species, possesses an unusual active site architecture that facilitates amine-activated conjugate alkene reduction followed by reductive amination. This enzyme can couple a broad selection of alpha,beta-unsaturated carbonyls with amines for the efficient preparation of chiral amine diastereomers bearing up to three stereocentres. Mechanistic and structural studies have been carried out to delineate the order of individual steps catalysed by EneIRED, which have led to a proposal for the overall catalytic cycle. This work shows that the IRED family can serve as a platform for facilitating the discovery of further enzymatic activities for application in synthetic biology and organic synthesis. | ||
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| + | Multifunctional biocatalyst for conjugate reduction and reductive amination.,Thorpe TW, Marshall JR, Harawa V, Ruscoe RE, Cuetos A, Finnigan JD, Angelastro A, Heath RS, Parmeggiani F, Charnock SJ, Howard RM, Kumar R, Daniels DSB, Grogan G, Turner NJ Nature. 2022 Apr;604(7904):86-91. doi: 10.1038/s41586-022-04458-x. Epub 2022 Apr , 6. PMID:35388195<ref>PMID:35388195</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7a3w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas putida]] | ||
[[Category: Cuetos A]] | [[Category: Cuetos A]] | ||
[[Category: Grogan G]] | [[Category: Grogan G]] | ||
[[Category: Thorpe T]] | [[Category: Thorpe T]] | ||
[[Category: Turner NJ]] | [[Category: Turner NJ]] | ||
Revision as of 07:41, 8 March 2023
Structure of Imine Reductase from Pseudomonas sp.
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