7duf
From Proteopedia
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==Crystal structure of VIM1 PHD finger.== | ==Crystal structure of VIM1 PHD finger.== | ||
| - | <StructureSection load='7duf' size='340' side='right'caption='[[7duf]]' scene=''> | + | <StructureSection load='7duf' size='340' side='right'caption='[[7duf]], [[Resolution|resolution]] 2.61Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DUF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7duf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DUF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7duf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7duf OCA], [https://pdbe.org/7duf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7duf RCSB], [https://www.ebi.ac.uk/pdbsum/7duf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7duf ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORTH2, VIM1, At1g57820, F12K22.14 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RING-type_E3_ubiquitin_transferase RING-type E3 ubiquitin transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.27 2.3.2.27] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7duf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7duf OCA], [https://pdbe.org/7duf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7duf RCSB], [https://www.ebi.ac.uk/pdbsum/7duf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7duf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/ORTH2_ARATH ORTH2_ARATH]] E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzyme UBC11. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Associates with methylated DNA, and can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate. Probably acts at the DNA methylation?histone interface to maintain centromeric heterochromatin.<ref>PMID:17239600</ref> <ref>PMID:17242155</ref> <ref>PMID:18643997</ref> <ref>PMID:18704160</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The PHD finger-containing VARIANT IN METHYLATION/ORTHRUS (VIM/ORTH) family of proteins in Arabidopsis consists of functional homologues of mammalian UHRF1 and is required for the maintenance of DNA methylation. Comparison of the sequence with those of other PHD fingers implied that VIM1 and VIM3 PHD could recognize lysine 4 of histone H3 (H3K4) through interactions mediated by a conserved aspartic acid. However, our calorimetric and modified histone peptide array binding studies suggested that neither H3K4 nor other histone marks are recognized by VIM1 and VIM3 PHD fingers. Here, we report a 2.6 A resolution crystal structure of the VIM1 PHD finger and demonstrate significant structural changes in the putative H3 recognition segments in contrast to canonical H3K4 binding PHD fingers. These changes include (i) the H3A1 binding region, (ii) strand beta1 that forms an intermolecular beta-sheet with the H3 peptide, and (iii) an aspartate-containing motif involved in salt bridge interaction with H3K4, which together appear to abrogate recognition of H3K4 by the VIM1 PHD finger. To understand the significance of the altered structural features in the VIM1 PHD that might prevent histone H3 recognition, we modeled a chimeric VIM1 PHD (chmVIM1 PHD) by grafting the peptide binding structural features of the BHC80 PHD onto the VIM1 PHD. Molecular dynamics simulation and metadynamics analyses revealed that the chmVIM1 PHD-H3 complex is stable and also showed a network of intermolecular interactions similar to those of the BHC80 PHD-H3 complex. Collectively, this study reveals that subtle structural changes in the peptide binding region of the VIM1 PHD abrogate histone H3 recognition. | ||
| + | |||
| + | Helical and beta-Turn Conformations in the Peptide Recognition Regions of the VIM1 PHD Finger Abrogate H3K4 Peptide Recognition.,Abhishek S, Deeksha W, Rajakumara E Biochemistry. 2021 Aug 18. doi: 10.1021/acs.biochem.1c00191. PMID:34404204<ref>PMID:34404204</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7duf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arath]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Abhishek S]] | + | [[Category: RING-type E3 ubiquitin transferase]] |
| - | [[Category: Deeksha W]] | + | [[Category: Abhishek, S]] |
| - | [[Category: Patel | + | [[Category: Deeksha, W]] |
| - | [[Category: Rajakumara E]] | + | [[Category: Patel, D J]] |
| + | [[Category: Rajakumara, E]] | ||
| + | [[Category: Arabidopsis]] | ||
| + | [[Category: Ligase]] | ||
| + | [[Category: Orthrus 2]] | ||
| + | [[Category: Phd finger]] | ||
| + | [[Category: Plant homeodomain]] | ||
| + | [[Category: Zinc finger]] | ||
Revision as of 10:51, 16 February 2022
Crystal structure of VIM1 PHD finger.
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