1f4k

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{{STRUCTURE_1f4k| PDB=1f4k | SCENE= }}
{{STRUCTURE_1f4k| PDB=1f4k | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX'''
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===CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX===
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==Overview==
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The coordinated termination of DNA replication is an important step in the life cycle of bacteria with circular chromosomes, but has only been defined at a molecular level in two systems to date. Here we report the structure of an engineered replication terminator protein (RTP) of Bacillus subtilis in complex with a 21 base pair DNA by X-ray crystallography at 2.5 A resolution. We also use NMR spectroscopic titration techniques. This work reveals a novel DNA interaction involving a dimeric 'winged helix' domain protein that differs from predictions. While the two recognition helices of RTP are in close contact with the B-form DNA major grooves, the 'wings' and N-termini of RTP do not form intimate contacts with the DNA. This structure provides insight into the molecular basis of polar replication fork arrest based on a model of cooperative binding and differential binding affinities of RTP to the two adjacent binding sites in the complete terminator.
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==About this Structure==
==About this Structure==
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[[Category: Replication and termination]]
[[Category: Replication and termination]]
[[Category: Winged-helix protein-dna complex]]
[[Category: Winged-helix protein-dna complex]]
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Revision as of 23:40, 30 June 2008

Image:1f4k.png

Template:STRUCTURE 1f4k

CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX

Template:ABSTRACT PUBMED 11224562

About this Structure

1F4K is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest., Wilce JA, Vivian JP, Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC, Nat Struct Biol. 2001 Mar;8(3):206-10. PMID:11224562

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