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1ntc

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SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF NTRC WITH THREE ALANINE SUBSTITUTIONS

Structural highlights

1ntc is a 2 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NTRC_SALTY Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of the 20 kDa C-terminal DNA-binding domain of NtrC from Salmonella typhimurium (residues Asp380-Glu469) with alanine replacing Arg456, Asn457, and Arg461, was determined by NMR spectroscopy. NtrC is a homodimeric enhancer-binding protein that activates the transcription of genes whose products are required for nitrogen metabolism. The 91-residue C-terminal domain contains the determinants necessary for dimerization and DNA-binding of the full length protein. The mutant protein does not bind to DNA but retains many characteristics of the wild-type protein, and the mutant domain expresses at high yield (20 mg/l) in minimal medium. Three-dimensional (1)H/(13)C/(15)N triple-resonance, (1)H-(13)C-(13)C-(1)H correlation and (15)N-separated nuclear Overhauser effect (NOE) spectroscopy experiments were used to make backbone and side-chain (1)H,(15)N, and (13)C assignments. The structures were calculated using a total of 1580 intra and inter-monomer distance and hydrogen bond restraints (88 hydrogen bonds; 44 hydrogen bond restraints), and 88 phi dihedral restraints for residues Asp400 through Glu469 in both monomers. A total of 54 ambiguous restraints (intra or inter-monomer) involving residues close to the 2-fold symmetry axis were also included. Each monomer consists of four helical segments. Helices A (Trp402-Leu414) and B (Leu421-His440) join with those of another monomer to form an antiparallel four-helix bundle. Helices C (Gln446-Leu451) and D (Ala456-Met468) of each monomer adopt a classic helix-turn-helix DNA-binding fold at either end of the protein. The backbone rms deviation for the 28 best of 40 starting structures is 0.6 (+/-0.2) A. Structural differences between the C-terminal domain of NtrC and the homologous Factor for Inversion Stimulation are discussed.

Solution structure of the DNA-binding domain of NtrC with three alanine substitutions.,Pelton JG, Kustu S, Wemmer DE J Mol Biol. 1999 Oct 8;292(5):1095-110. PMID:10512705^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pelton JG, Kustu S, Wemmer DE. Solution structure of the DNA-binding domain of NtrC with three alanine substitutions. J Mol Biol. 1999 Oct 8;292(5):1095-110. PMID:10512705 doi:10.1006/jmbi.1999.3140

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ntc"

Categories: Large Structures | Salmonella enterica subsp. enterica serovar Typhimurium | Kustu S | Pelton JG | Wemmer DE

@@ Line 1: / Line 1: @@
 ==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF NTRC WITH THREE ALANINE SUBSTITUTIONS==
-<StructureSection load='1ntc' size='340' side='right'caption='[[1ntc]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''>
+<StructureSection load='1ntc' size='340' side='right'caption='[[1ntc]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ntc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ntc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTC FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntc OCA], [https://pdbe.org/1ntc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntc RCSB], [https://www.ebi.ac.uk/pdbsum/1ntc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntc ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntc OCA], [https://pdbe.org/1ntc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntc RCSB], [https://www.ebi.ac.uk/pdbsum/1ntc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntc ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/NTRC_SALTY NTRC_SALTY]] Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.
+[https://www.uniprot.org/uniprot/NTRC_SALTY NTRC_SALTY] Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Kustu, S]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-[[Category: Pelton, J G]]
+[[Category: Kustu S]]
-[[Category: Wemmer, D E]]
+[[Category: Pelton JG]]
-[[Category: Fi]]
+[[Category: Wemmer DE]]
-[[Category: Four-helix bundle]]
-[[Category: Helix-turn-helix]]
-[[Category: Transcription regulation]]

1ntc

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Current revision

SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF NTRC WITH THREE ALANINE SUBSTITUTIONS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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