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7me1
From Proteopedia
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== Function == | == Function == | ||
[[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE]] Part of an ATP-driven transport system YfeABCD for chelated iron. | [[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE]] Part of an ATP-driven transport system YfeABCD for chelated iron. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 A resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from approximately 4 to approximately 16 A away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination. | ||
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| + | Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site.,Radka CD, Aller SG Acta Crystallogr F Struct Biol Commun. 2021 Sep 1;77(Pt 9):286-293. doi:, 10.1107/S2053230X21008086. Epub 2021 Aug 24. PMID:34473105<ref>PMID:34473105</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7me1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 09:37, 15 September 2021
YfeA oligomer crystal 1, form 1
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