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1f6g

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[[Image:1f6g.gif|left|200px]]
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[[Image:1f6g.png|left|200px]]
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==Overview==
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{{ABSTRACT_11158168}}
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The molecular architecture of the NH(2) and COOH termini of the prokaryotic potassium channel KcsA has been determined using site-directed spin-labeling methods and paramagnetic resonance EPR spectroscopy. Cysteine mutants were generated (residues 5-24 and 121-160) and spin labeled, and the X-band CW EPR spectra were obtained from liposome-reconstituted channels at room temperature. Data on probe mobility (DeltaHo(-1)), accessibility parameters (PiO(2) and PiNiEdda), and inter-subunit spin-spin interaction (Omega) were used as structural constraints to build a three-dimensional folding model of these cytoplasmic domains from a set of simulated annealing and restrained molecular dynamics runs. 32 backbone structures were generated and averaged using fourfold symmetry, and a final mean structure was obtained from the eight lowest energy runs. Based on the present data, together with information from the KcsA crystal structure, a model for the three-dimensional fold of full-length KcsA was constructed. In this model, the NH(2) terminus of KcsA forms an alpha-helix anchored at the membrane-water interface, while the COOH terminus forms a right-handed four-helix bundle that extend some 40-50 A towards the cytoplasm. Functional analysis of COOH-terminal deletion constructs suggest that, while the COOH terminus does not play a substantial role in determining ion permeation properties, it exerts a modulatory role in the pH-dependent gating mechanism.
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==About this Structure==
==About this Structure==
1F6G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. The following page contains interesting information on the relation of 1F6G with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6G OCA].
1F6G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. The following page contains interesting information on the relation of 1F6G with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6G OCA].
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==Reference==
 
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Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating., Cortes DM, Cuello LG, Perozo E, J Gen Physiol. 2001 Feb;117(2):165-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11158168 11158168]
 
[[Category: Potassium Channels]]
[[Category: Potassium Channels]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Integral membrane protein]]
[[Category: Integral membrane protein]]
[[Category: Potassium channel]]
[[Category: Potassium channel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:57:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:15:23 2008''

Revision as of 14:15, 26 June 2008

Image:1f6g.png

Template:STRUCTURE 1f6g

POTASSIUM CHANNEL (KCSA) FULL-LENGTH FOLD


Template:ABSTRACT 11158168

About this Structure

1F6G is a Single protein structure of sequence from Streptomyces lividans. The following page contains interesting information on the relation of 1F6G with [Potassium Channels]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:15:23 2008

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