1pwo
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='1pwo' size='340' side='right'caption='[[1pwo]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='1pwo' size='340' side='right'caption='[[1pwo]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1pwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1pwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Micropechis_ikaheca Micropechis ikaheca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PWO FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pwo OCA], [https://pdbe.org/1pwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pwo RCSB], [https://www.ebi.ac.uk/pdbsum/1pwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pwo ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pwo OCA], [https://pdbe.org/1pwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pwo RCSB], [https://www.ebi.ac.uk/pdbsum/1pwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pwo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| Line 17: | Line 17: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pwo ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pwo ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. The C-termini of the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster together, forming a highly exposed area. A BLAST search using the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s. | ||
| - | |||
| - | Structure and function comparison of Micropechis ikaheka snake venom phospholipase A2 isoenzymes.,Lok SM, Gao R, Rouault M, Lambeau G, Gopalakrishnakone P, Swaminathan K FEBS J. 2005 Mar;272(5):1211-20. PMID:15720395<ref>PMID:15720395</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1pwo" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]] | *[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Micropechis | + | [[Category: Micropechis ikaheca]] |
| - | [[Category: Lok | + | [[Category: Lok SM]] |
| - | [[Category: Swaminathan | + | [[Category: Swaminathan K]] |
| - | + | ||
| - | + | ||
| - | + | ||
Revision as of 06:11, 3 April 2024
Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka
| |||||||||||
