7me6

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Current revision (07:18, 3 April 2024) (edit) (undo)
 
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<StructureSection load='7me6' size='340' side='right'caption='[[7me6]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
<StructureSection load='7me6' size='340' side='right'caption='[[7me6]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7me6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7me6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.053&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ycnI, BSU03940 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me6 OCA], [https://pdbe.org/7me6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me6 RCSB], [https://www.ebi.ac.uk/pdbsum/7me6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me6 OCA], [https://pdbe.org/7me6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me6 RCSB], [https://www.ebi.ac.uk/pdbsum/7me6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me6 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/YCNI_BACSU YCNI_BACSU]
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Bacteria require a precise balance of copper ions to ensure that essential cuproproteins are fully metalated while also avoiding copper-induced toxicity. The Gram-positive bacterium Bacillus subtilis maintains appropriate copper homeostasis in part through the ycn operon. The ycn operon comprises genes encoding three proteins: the putative copper importer YcnJ, the copper-dependent transcriptional repressor YcnK, and the uncharacterized Domain of Unknown Function 1775 (DUF1775) containing YcnI. DUF1775 domains are found across bacterial phylogeny, and bioinformatics analyses indicate that they frequently neighbor domains implicated in copper homeostasis and transport. Here, we investigated whether YcnI can interact with copper and, using electron paramagnetic resonance and inductively coupled plasma-MS, found that this protein can bind a single Cu(II) ion. We determine the structure of both the apo and copper-bound forms of the protein by X-ray crystallography, uncovering a copper-binding site featuring a unique monohistidine brace ligand set that is highly conserved among DUF1775 domains. These data suggest a possible role for YcnI as a copper chaperone and that DUF1775 domains in other bacterial species may also function in copper homeostasis.
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The YcnI protein from Bacillus subtilis contains a copper-binding domain.,Damle MS, Singh AN, Peters SC, Szalai VA, Fisher OS J Biol Chem. 2021 Aug 14;297(3):101078. doi: 10.1016/j.jbc.2021.101078. PMID:34400169<ref>PMID:34400169</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7me6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Vibrio subtilis ehrenberg 1835]]
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[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Damle, M]]
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[[Category: Damle M]]
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[[Category: Fisher, O S]]
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[[Category: Fisher OS]]
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[[Category: Cu-binding]]
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[[Category: Cupredoxin]]
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[[Category: Metal binding protein]]
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Current revision

Structure of the apo form of YcnI

PDB ID 7me6

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OCA

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