7bid
From Proteopedia
(Difference between revisions)
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==Crystal structure of v31WRAP-T, a 7-bladed designer protein== | ==Crystal structure of v31WRAP-T, a 7-bladed designer protein== | ||
| - | <StructureSection load='7bid' size='340' side='right'caption='[[7bid]]' scene=''> | + | <StructureSection load='7bid' size='340' side='right'caption='[[7bid]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BID FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bid]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BID FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bid OCA], [https://pdbe.org/7bid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bid RCSB], [https://www.ebi.ac.uk/pdbsum/7bid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bid ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bid OCA], [https://pdbe.org/7bid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bid RCSB], [https://www.ebi.ac.uk/pdbsum/7bid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bid ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | [Formula: see text]-Propeller proteins are common natural disc-like pseudo-symmetric proteins that contain multiple repeats ('blades') each consisting of a 4-stranded anti-parallel [Formula: see text]-sheet. So far, 4- to 12-bladed [Formula: see text]-propellers have been discovered in nature showing large functional and sequential variation. Using computational design approaches, we created perfectly symmetric [Formula: see text]-propellers out of natural pseudo-symmetric templates. These proteins are useful tools to study protein evolution of this very diverse fold. While the 7-bladed architecture is the most common, no symmetric 7-bladed monomer has been created and characterized so far. Here we describe such a engineered protein, based on a highly symmetric natural template, and test the effects of circular permutation on its stability. Geometrical analysis of this protein and other artificial symmetrical proteins reveals no systematic constraint that could be used to help in engineering of this fold, and suggests sequence constraints unique to each [Formula: see text]-propeller sub-family. | ||
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| + | Structure and stability of the designer protein WRAP-T and its permutants.,Mylemans B, Lee XY, Laier I, Helsen C, Voet ARD Sci Rep. 2021 Sep 22;11(1):18867. doi: 10.1038/s41598-021-98391-0. PMID:34552189<ref>PMID:34552189</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bid" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Laier I]] | + | [[Category: Laier, I]] |
| - | [[Category: Lee | + | [[Category: Lee, X Y]] |
| - | [[Category: Mylemans B]] | + | [[Category: Mylemans, B]] |
| - | [[Category: Voet | + | [[Category: Voet, A R.D]] |
| + | [[Category: De novo protein]] | ||
| + | [[Category: Synthetic construct]] | ||
Revision as of 05:51, 6 October 2021
Crystal structure of v31WRAP-T, a 7-bladed designer protein
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