7bif
From Proteopedia
(Difference between revisions)
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==Crystal structure of v22WRAP-T, a 7-bladed designer protein== | ==Crystal structure of v22WRAP-T, a 7-bladed designer protein== | ||
| - | <StructureSection load='7bif' size='340' side='right'caption='[[7bif]]' scene=''> | + | <StructureSection load='7bif' size='340' side='right'caption='[[7bif]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bif]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BIF FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bif OCA], [https://pdbe.org/7bif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bif RCSB], [https://www.ebi.ac.uk/pdbsum/7bif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bif ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bif OCA], [https://pdbe.org/7bif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bif RCSB], [https://www.ebi.ac.uk/pdbsum/7bif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bif ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | [Formula: see text]-Propeller proteins are common natural disc-like pseudo-symmetric proteins that contain multiple repeats ('blades') each consisting of a 4-stranded anti-parallel [Formula: see text]-sheet. So far, 4- to 12-bladed [Formula: see text]-propellers have been discovered in nature showing large functional and sequential variation. Using computational design approaches, we created perfectly symmetric [Formula: see text]-propellers out of natural pseudo-symmetric templates. These proteins are useful tools to study protein evolution of this very diverse fold. While the 7-bladed architecture is the most common, no symmetric 7-bladed monomer has been created and characterized so far. Here we describe such a engineered protein, based on a highly symmetric natural template, and test the effects of circular permutation on its stability. Geometrical analysis of this protein and other artificial symmetrical proteins reveals no systematic constraint that could be used to help in engineering of this fold, and suggests sequence constraints unique to each [Formula: see text]-propeller sub-family. | ||
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| + | Structure and stability of the designer protein WRAP-T and its permutants.,Mylemans B, Lee XY, Laier I, Helsen C, Voet ARD Sci Rep. 2021 Sep 22;11(1):18867. doi: 10.1038/s41598-021-98391-0. PMID:34552189<ref>PMID:34552189</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bif" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Laier I]] | + | [[Category: Laier, I]] |
| - | [[Category: Lee | + | [[Category: Lee, X Y]] |
| - | [[Category: Mylemans B]] | + | [[Category: Mylemans, B]] |
| - | [[Category: Voet | + | [[Category: Voet, A R.D]] |
| + | [[Category: De novo protein]] | ||
| + | [[Category: Synthetic construct]] | ||
Revision as of 05:51, 6 October 2021
Crystal structure of v22WRAP-T, a 7-bladed designer protein
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