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1fah

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{{STRUCTURE_1fah| PDB=1fah | SCENE= }}
{{STRUCTURE_1fah| PDB=1fah | SCENE= }}
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'''STRUCTURE OF CYTOCHROME P450'''
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===STRUCTURE OF CYTOCHROME P450===
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==Overview==
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Cytochrome P450BM-3, a catalytically self-sufficient monooxygenase from Bacillus megaterium, catalyzes the omega-n (n = 1-3) hydroxylation of fatty acids in the presence of O2 and NADPH. Like most other P450s, cytochrome P450BM-3 contains a threonine residue (Thr268) in the distal I helix thought to be important for O2 binding and activation. Thr268 has been converted to alanine and the enzymatic properties and heme domain crystal structure determined. Using sodium laurate as the substrate, the mutant exhibited slower rates of O2 and NADPH consumption. In addition, electron transfer is uncoupled from substrate hydroxylation as evidenced by the greater production of water and peroxide in the mutant compared to the wild-type enzyme. The crystal structure of the mutant reveals that the only changes in structure are confined to the site of mutation. These data indicate an important role for Thr268 in O2 binding and activation in the metabolism of sodium laurate by cytochrome P450BM-3.
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(as it appears on PubMed at http://www.pubmed.gov), where 7578081 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7578081}}
==About this Structure==
==About this Structure==
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[[Category: Heme]]
[[Category: Heme]]
[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:06:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:57:31 2008''

Revision as of 23:57, 30 June 2008

Image:1fah.png

Template:STRUCTURE 1fah

STRUCTURE OF CYTOCHROME P450

Template:ABSTRACT PUBMED 7578081

About this Structure

1FAH is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.

Reference

The role of Thr268 in oxygen activation of cytochrome P450BM-3., Yeom H, Sligar SG, Li H, Poulos TL, Fulco AJ, Biochemistry. 1995 Nov 14;34(45):14733-40. PMID:7578081

Page seeded by OCA on Tue Jul 1 02:57:31 2008

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