7cze
From Proteopedia
(Difference between revisions)
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- | ==== | + | ==Crystal structure of Epstein-Barr virus (EBV) gHgL and in complex with the ligand binding domian (LBD) of EphA2== |
- | <StructureSection load='7cze' size='340' side='right'caption='[[7cze]]' scene=''> | + | <StructureSection load='7cze' size='340' side='right'caption='[[7cze]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7cze]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CZE FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cze OCA], [https://pdbe.org/7cze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cze RCSB], [https://www.ebi.ac.uk/pdbsum/7cze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cze ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cze OCA], [https://pdbe.org/7cze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cze RCSB], [https://www.ebi.ac.uk/pdbsum/7cze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cze ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/GH_EBVB9 GH_EBVB9] The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. Fusion of EBV with B-lymphocytes requires the additional receptor-binding protein gp42, which forms a complex with gH/gL. May also be required for virus attachment to epithelial cells.<ref>PMID:11021994</ref> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | The human gamma-herpesviruses Kaposi sarcoma associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) are associated with many human malignancies. Viral glycoprotein H (gH) and glycoprotein L (gL) are crucial for the cell tropism by binding to specific receptors. Recently, EphA2 was identified as the specific entry receptor for both KSHV and EBV. Here, we characterized the crystal structures of KSHV gHgL or EBV gHgL in complex with the ligand binding domain (LBD) of EphA2. Both KSHV and EBV gHgL bind to the channel and peripheral regions of LBD primarily using gL. Extensive interactions with more contacts contribute to the higher affinity of KSHV gHgL to LBD than that of EBV gHgL. These binding characteristics were verified using cell-based fusion assays with mutations in key EphA2 residues. Our experiments suggest that multiple animal gamma-herpesviruses could use EphA2 as an entry receptor, implying a potential threat to human health. | ||
+ | |||
+ | Molecular basis of EphA2 recognition by gHgL from gammaherpesviruses.,Su C, Wu L, Chai Y, Qi J, Tan S, Gao GF, Song H, Yan J Nat Commun. 2020 Nov 24;11(1):5964. doi: 10.1038/s41467-020-19617-9. PMID:33235207<ref>PMID:33235207</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7cze" style="background-color:#fffaf0;"></div> | ||
+ | |||
+ | ==See Also== | ||
+ | *[[Ephrin receptor 3D structures|Ephrin receptor 3D structures]] | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Homo sapiens]] | ||
+ | [[Category: Human herpesvirus 4 strain B95-8]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
- | [[Category: | + | [[Category: Chai Y]] |
+ | [[Category: Gao GF]] | ||
+ | [[Category: Qi JX]] | ||
+ | [[Category: Song H]] | ||
+ | [[Category: Su C]] | ||
+ | [[Category: Wu LL]] | ||
+ | [[Category: Yan JH]] |
Revision as of 16:20, 29 November 2023
Crystal structure of Epstein-Barr virus (EBV) gHgL and in complex with the ligand binding domian (LBD) of EphA2
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Categories: Homo sapiens | Human herpesvirus 4 strain B95-8 | Large Structures | Chai Y | Gao GF | Qi JX | Song H | Su C | Wu LL | Yan JH