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1ue1

From Proteopedia

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Current revision

Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis

Structural highlights

1ue1 is a 2 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

SSB_MYCTU This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Single-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB.

Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications.,Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M. Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications. J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ue1"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ue1' size='340' side='right'caption='[[1ue1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ue1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UE1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ue1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UE1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ue5|1ue5]], [[1ue6|1ue6]], [[1ue7|1ue7]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ue1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ue1 OCA], [https://pdbe.org/1ue1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ue1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ue1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ue1 ProSAT], [https://www.topsan.org/Proteins/TBSGC/1ue1 TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/SSB_MYCTU SSB_MYCTU]] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).
+[https://www.uniprot.org/uniprot/SSB_MYCTU SSB_MYCTU] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Acharya, N]]
+[[Category: Mycobacterium tuberculosis]]
-[[Category: Jeyakanthan, J]]
+[[Category: Acharya N]]
-[[Category: Saikrishnan, K]]
+[[Category: Jeyakanthan J]]
-[[Category: Sekar, K]]
+[[Category: Saikrishnan K]]
-[[Category: Structural genomic]]
+[[Category: Sekar K]]
-[[Category: Varshney, U]]
+[[Category: Varshney U]]
-[[Category: Venkatesh, J]]
+[[Category: Venkatesh J]]
-[[Category: Vijayan, M]]
+[[Category: Vijayan M]]
-[[Category: Dna binding protein]]
-[[Category: Dna-binding protein]]
-[[Category: Oligonucleotide binding fold]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Tbsgc]]

1ue1

From Proteopedia

Current revision

Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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