1wbc

From Proteopedia

(Difference between revisions)

Revision as of 13:33, 9 May 2024

CRYSTALLIZATION AND PRELIMINARY X-RAY STUDIES OF PSOPHOCARPIN B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN SEEDS

Structural highlights

1wbc is a 1 chain structure with sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.95Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ICW3_PSOTE Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Thc crystal structure of an alpha-chymotrypsin inhibitor (P6(1)22; a = 61.4, c = 210.9 A) isolated from winged bean (Psophocarpus. tetragonolobus) seeds has been determined at 2.95 A resolution by the molecular-replacement method using the 2.6 A coordinates of Erythrina trypsin inhibitor (ETI) as the starting model (57% sequence homology). This protease inhibitor, WCI, belongs to the Kunitz (STI) family and is a single polypeptide chain with 183 amino-acid residues having a molecular weight of 20 244 Da. Structure refinement with RESTRAIN and X-PLOR has led to a crystallographic R factor of 19.1% for 3469 observed reflections (I > 2sigma) in the resolution range 8-2.95 A. A total of 56 water molecules have been incorporated in the refined model containing 181 amino-acid residues. In the refined structure the deviations of bond lengths and bond angles from ideal values are 0.015 A and 2.2 degrees, respectively. The inhibitor molecule is spherical and consists of 12 antiparallel beta-strands with connecting loops arranged in a characteristic folding (a six-stranded beta-barrel and a six-stranded lid on one hollow end of the barrel) common to other homologous serine protease inhibitors in the Kunitz (STI) family as well as to some non-homologous proteins like interleukin-lalpha and interleukin-lbeta. In the structure the conformation of the protruding reactive-site loop is stabilized through hydrogen bonds mainly formed by the side chain of Asnl4, which intrudes inside the cavity of the reactive-site loop, with the side-chain and main-chain atoms of some residues in the loop region. A pseudo threefold axis exists parallel to the barrel axis of the structure. Each of the three subdomains comprises of four beta-strands with connecting loops.

Structure of a Kunitz-type chymotrypsin from winged bean seeds at 2.95 A resolution.,Dattagupta JK, Podder A, Chakrabarti C, Sen U, Dutta SK, Singh M Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):521-8. PMID:15299674^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dattagupta JK, Podder A, Chakrabarti C, Sen U, Dutta SK, Singh M. Structure of a Kunitz-type chymotrypsin from winged bean seeds at 2.95 A resolution. Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):521-8. PMID:15299674 doi:10.1107/S0907444996000224

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wbc"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1wbc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WBC FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbc OCA], [https://pdbe.org/1wbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wbc RCSB], [https://www.ebi.ac.uk/pdbsum/1wbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbc ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbc OCA], [https://pdbe.org/1wbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wbc RCSB], [https://www.ebi.ac.uk/pdbsum/1wbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbc ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
+[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 37: @@
 [[Category: Large Structures]]
 [[Category: Psophocarpus tetragonolobus]]
-[[Category: Chakrabarti, C]]
+[[Category: Chakrabarti C]]
-[[Category: Dattagupta, J K]]
+[[Category: Dattagupta JK]]
-[[Category: Dutta, S K]]
+[[Category: Dutta SK]]
-[[Category: Podder, A]]
+[[Category: Podder A]]
-[[Category: Sen, U]]
+[[Category: Sen U]]
-[[Category: Singh, M]]
+[[Category: Singh M]]
-[[Category: Serine protease inhibitor]]

1wbc

From Proteopedia

Revision as of 13:33, 9 May 2024

CRYSTALLIZATION AND PRELIMINARY X-RAY STUDIES OF PSOPHOCARPIN B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN SEEDS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox