1c9b
From Proteopedia
(New page: 200px<br /> <applet load="1c9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c9b, resolution 2.65Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1c9b.gif|left|200px]]<br /> | + | [[Image:1c9b.gif|left|200px]]<br /><applet load="1c9b" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1c9b" size=" | + | |
caption="1c9b, resolution 2.65Å" /> | caption="1c9b, resolution 2.65Å" /> | ||
'''CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)'''<br /> | '''CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transcription initiation requires the assembly of a preinitiation complex | + | Transcription initiation requires the assembly of a preinitiation complex (PIC), which is nucleated through binding of the TATA-box binding protein (TBP) to the promoter. Biochemical studies have shown, however, that TBP recognizes the TATA-box in both orientations and, therefore, cannot account for the directionality of PIC assembly. Transcription factor IIB (TFIIB) is essential for transcription initiation from RNA polymerase II promoters. Recent functional studies have identified a specific 7 bp TFIIB recognition element (BRE) immediately upstream of the TATA-box. We present here the 2.65 A resolution crystal structure of a human TFIIBc-TBPc complex bound to an idealized and extended adenovirus major late promoter. This structure now reveals that human TFIIBc binds to the promoter asymmetrically through base-specific contacts in the major groove upstream and in the minor groove downstream of the TATA-box. Binding of TFIIBc is, therefore, synergistic with TBPc requiring the distortion of the TATA-box. Thus, the newly described TFIIBc-DNA interface is likely to be a key determinant for the unidirectional assembly of a functional PIC. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1C9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1C9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Sigler, P | + | [[Category: Sigler, P B.]] |
| - | [[Category: Tsai, F | + | [[Category: Tsai, F T.F.]] |
[[Category: cyclin-like fold]] | [[Category: cyclin-like fold]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
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[[Category: tata-box]] | [[Category: tata-box]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:03:44 2008'' |
Revision as of 10:03, 21 February 2008
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CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)
Contents |
Overview
Transcription initiation requires the assembly of a preinitiation complex (PIC), which is nucleated through binding of the TATA-box binding protein (TBP) to the promoter. Biochemical studies have shown, however, that TBP recognizes the TATA-box in both orientations and, therefore, cannot account for the directionality of PIC assembly. Transcription factor IIB (TFIIB) is essential for transcription initiation from RNA polymerase II promoters. Recent functional studies have identified a specific 7 bp TFIIB recognition element (BRE) immediately upstream of the TATA-box. We present here the 2.65 A resolution crystal structure of a human TFIIBc-TBPc complex bound to an idealized and extended adenovirus major late promoter. This structure now reveals that human TFIIBc binds to the promoter asymmetrically through base-specific contacts in the major groove upstream and in the minor groove downstream of the TATA-box. Binding of TFIIBc is, therefore, synergistic with TBPc requiring the distortion of the TATA-box. Thus, the newly described TFIIBc-DNA interface is likely to be a key determinant for the unidirectional assembly of a functional PIC.
Disease
Known diseases associated with this structure: Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1C9B is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of preinitiation complex assembly on human pol II promoters., Tsai FT, Sigler PB, EMBO J. 2000 Jan 4;19(1):25-36. PMID:10619841
Page seeded by OCA on Thu Feb 21 12:03:44 2008
