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3hcq

From Proteopedia

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Structural analysis of the choline binding protein ChoX in a semi-closed and ligand-free conformation

Structural highlights

3hcq is a 2 chain structure with sequence from Sinorhizobium meliloti. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.89Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q92N37_RHIME

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed but substrate-free state of ChoX. This latter structure revealed an architecture of the ligand-binding site that is superimposable to the closed, ligand-bound form of ChoX. We report here the crystal structure of ChoX in an unusual, ligand-free conformation that represents a semi-closed form of ChoX. The analysis revealed a subdomain movement in the N-lobe of ChoX. Comparison with the two well-characterized substrate binding proteins, MBP and HisJ, suggests the presence of a similar subdomain in these proteins.

Structural analysis of the choline-binding protein ChoX in a semi-closed and ligand-free conformation.,Oswald C, Smits SH, Hoing M, Bremer E, Schmitt L Biol Chem. 2009 Nov;390(11):1163-70. PMID:19642870^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oswald C, Smits SH, Hoing M, Bremer E, Schmitt L. Structural analysis of the choline-binding protein ChoX in a semi-closed and ligand-free conformation. Biol Chem. 2009 Nov;390(11):1163-70. PMID:19642870 doi:10.1515/BC.2009.113

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hcq"

@@ Line 3: / Line 3: @@
 <StructureSection load='3hcq' size='340' side='right'caption='[[3hcq]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3hcq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HCQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3hcq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HCQ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2reg|2reg]], [[2rin|2rin]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">choX, R02399, SMc02737 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=382 ATCC 9930])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hcq OCA], [https://pdbe.org/3hcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hcq RCSB], [https://www.ebi.ac.uk/pdbsum/3hcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hcq ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q92N37_RHIME Q92N37_RHIME]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 9930]]
 [[Category: Large Structures]]
-[[Category: Bremer, E]]
+[[Category: Sinorhizobium meliloti]]
-[[Category: Hoeing, M]]
+[[Category: Bremer E]]
-[[Category: Oswald, C]]
+[[Category: Hoeing M]]
-[[Category: Schmitt, L]]
+[[Category: Oswald C]]
-[[Category: Smits, S H.J]]
+[[Category: Schmitt L]]
-[[Category: Abc transporter]]
+[[Category: Smits SHJ]]
-[[Category: Binding protein]]
-[[Category: Choline-binding protein]]
-[[Category: Semi-closed]]
-[[Category: Transport]]

3hcq

From Proteopedia

Current revision

Structural analysis of the choline binding protein ChoX in a semi-closed and ligand-free conformation

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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