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| | <StructureSection load='2bh8' size='340' side='right'caption='[[2bh8]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='2bh8' size='340' side='right'caption='[[2bh8]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bh8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BH8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2bh8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BH8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mjc|1mjc]], [[3mef|3mef]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bh8 OCA], [https://pdbe.org/2bh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2bh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bh8 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bh8 OCA], [https://pdbe.org/2bh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2bh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bh8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CSPA_ECOLI CSPA_ECOLI] Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3'. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bono, S De]] | + | [[Category: De Bono S]] |
| - | [[Category: Girard, E]] | + | [[Category: Girard E]] |
| - | [[Category: Riechmann, L]] | + | [[Category: Riechmann L]] |
| - | [[Category: Williams, R L]] | + | [[Category: Williams RL]] |
| - | [[Category: Winter, G]] | + | [[Category: Winter G]] |
| - | [[Category: Activator]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Molecular evolution]]
| + | |
| - | [[Category: Ob-fold]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Ribosomal protein]]
| + | |
| - | [[Category: Rna- binding]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Unique architecture]]
| + | |
| Structural highlights
Function
CSPA_ECOLI Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3'.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
It has been suggested that protein domains evolved by the non-homologous recombination of building blocks of subdomain size. In earlier work we attempted to recapitulate domain evolution in vitro. We took a polypeptide segment comprising three beta-strands in the monomeric, five-stranded beta-barrel cold shock protein (CspA) of Escherichia coli as a building block. This segment corresponds to a complete exon in homologous eukaryotic proteins and includes residues that nucleate folding in CspA. We recombined this segment at random with fragments of natural proteins and succeeded in generating a range of folded chimaeric proteins. We now present the crystal structure of one such combinatorial protein, 1b11, a 103-residue polypeptide that includes segments from CspA and the S1 domain of the 30S ribosomal subunit of E. coli. The structure reveals a segment-swapped, six-stranded beta-barrel of unique architecture that assembles to a tetramer. Surprisingly, the CspA segment retains its structural identity in 1b11, recapitulating its original fold and deforming the structure of the S1 segment as necessary to complete a barrel. Our work provides structural evidence that (i) random shuffling of nonhomologous polypeptide segments can lead to folded proteins and unique architectures, (ii) many structural features of the segments are retained, and (iii) some segments can act as templates around which the rest of the protein folds.
A segment of cold shock protein directs the folding of a combinatorial protein.,de Bono S, Riechmann L, Girard E, Williams RL, Winter G Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1396-401. Epub 2005 Jan 25. PMID:15671167[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ de Bono S, Riechmann L, Girard E, Williams RL, Winter G. A segment of cold shock protein directs the folding of a combinatorial protein. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1396-401. Epub 2005 Jan 25. PMID:15671167
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