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7eea
From Proteopedia
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==Cyanophage Pam1 tailspike receptor-binding domain== | ==Cyanophage Pam1 tailspike receptor-binding domain== | ||
| - | <StructureSection load='7eea' size='340' side='right'caption='[[7eea]]' scene=''> | + | <StructureSection load='7eea' size='340' side='right'caption='[[7eea]], [[Resolution|resolution]] 2.67Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7eea]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEA FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eea OCA], [https://pdbe.org/7eea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eea RCSB], [https://www.ebi.ac.uk/pdbsum/7eea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eea ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eea OCA], [https://pdbe.org/7eea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eea RCSB], [https://www.ebi.ac.uk/pdbsum/7eea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eea ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-A-long tail and an icosahedral capsid of 650 A in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a beta-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications. | ||
| + | |||
| + | Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1.,Zhang JT, Yang F, Du K, Li WF, Chen Y, Jiang YL, Li Q, Zhou CZ Structure. 2022 Feb 3;30(2):240-251.e4. doi: 10.1016/j.str.2021.10.004. Epub 2021, Nov 1. PMID:34727518<ref>PMID:34727518</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7eea" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jiang | + | [[Category: Jiang, Y L]] |
| - | [[Category: Zhang | + | [[Category: Zhang, J T]] |
| - | [[Category: Zhou | + | [[Category: Zhou, C Z]] |
| + | [[Category: Tailspike receptor-binding domain]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 13:03, 4 May 2022
Cyanophage Pam1 tailspike receptor-binding domain
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