3foa

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the bacteriophage T4 tail sheath protein, deletion mutant gp18M

Structural highlights

3foa is a 4 chain structure with sequence from Escherichia virus T4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSP_BPT4 Structural component of the bacteriophage tail which consists of a contractile sheath, a tube and a baseplate. The central cylindrical segment of the tail consists of a rigid tube, composed of multiple copies of gp19, surrounded by the outer contractile sheath assembled from gp18 subunits. A total of 138 copies of gp18 arranged into 23 hexameric rings constitutes the sheath. During infection, contraction of the sheath drives the central tube through the host outer membrane, creating a channel for DNA ejection from the capsid into the host cell.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.

The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria.,Aksyuk AA, Leiman PG, Kurochkina LP, Shneider MM, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG EMBO J. 2009 Apr 8;28(7):821-9. Epub 2009 Feb 19. PMID:19229296^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Leiman PG, Kanamaru S, Mesyanzhinov VV, Arisaka F, Rossmann MG. Structure and morphogenesis of bacteriophage T4. Cell Mol Life Sci. 2003 Nov;60(11):2356-70. PMID:14625682 doi:http://dx.doi.org/10.1007/s00018-003-3072-1
↑ Aksyuk AA, Leiman PG, Kurochkina LP, Shneider MM, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG. The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. EMBO J. 2009 Apr 8;28(7):821-9. Epub 2009 Feb 19. PMID:19229296 doi:http://dx.doi.org/10.1038/emboj.2009.36

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3foa"

@@ Line 3: / Line 3: @@
 <StructureSection load='3foa' size='340' side='right'caption='[[3foa]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3foa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3foa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOA FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fo8|3fo8]], [[3foh|3foh]], [[3foi|3foi]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">18, GB AAA32541 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3foa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3foa OCA], [https://pdbe.org/3foa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3foa RCSB], [https://www.ebi.ac.uk/pdbsum/3foa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3foa ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/VG18_BPT4 VG18_BPT4]] The contractile tail of bacteriophage T4 consists of a contractile sheath, a tube and a baseplate. 144 protomers of Gp18, arranged in 24 annuli, form the contractile tail sheath which participates in viral DNA injection into host cytoplasm.
+[https://www.uniprot.org/uniprot/TSP_BPT4 TSP_BPT4] Structural component of the bacteriophage tail which consists of a contractile sheath, a tube and a baseplate. The central cylindrical segment of the tail consists of a rigid tube, composed of multiple copies of gp19, surrounded by the outer contractile sheath assembled from gp18 subunits. A total of 138 copies of gp18 arranged into 23 hexameric rings constitutes the sheath. During infection, contraction of the sheath drives the central tube through the host outer membrane, creating a channel for DNA ejection from the capsid into the host cell.<ref>PMID:14625682</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bpt4]]
+[[Category: Escherichia virus T4]]
 [[Category: Large Structures]]
-[[Category: Aksyuk, A A]]
+[[Category: Aksyuk AA]]
-[[Category: Kostyuchenko, V A]]
+[[Category: Kostyuchenko VA]]
-[[Category: Kurochkina, L P]]
+[[Category: Kurochkina LP]]
-[[Category: Leiman, P G]]
+[[Category: Leiman PG]]
-[[Category: Mesyanzhinov, V V]]
+[[Category: Mesyanzhinov VV]]
-[[Category: Rossmann, M G]]
+[[Category: Rossmann MG]]
-[[Category: Shneider, M M]]
+[[Category: Shneider MM]]
-[[Category: Alpha-beta]]
-[[Category: Bacteriophage t4]]
-[[Category: Tail sheath]]
-[[Category: Viral protein]]
-[[Category: Viral structural protein]]

3foa

From Proteopedia

Current revision

Crystal structure of the bacteriophage T4 tail sheath protein, deletion mutant gp18M

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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