7vg9
From Proteopedia
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==Crystal structure of phosphotransbutyrylase from Clostridium acetobutylicum== | ==Crystal structure of phosphotransbutyrylase from Clostridium acetobutylicum== | ||
- | <StructureSection load='7vg9' size='340' side='right'caption='[[7vg9]]' scene=''> | + | <StructureSection load='7vg9' size='340' side='right'caption='[[7vg9]], [[Resolution|resolution]] 2.91Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VG9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vg9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VG9 FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vg9 OCA], [https://pdbe.org/7vg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vg9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vg9 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91Å</td></tr> |
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vg9 OCA], [https://pdbe.org/7vg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vg9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vg9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/PTB_CLOAB PTB_CLOAB] Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate. | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Acetone-butanol-ethanol (ABE) fermentation by the anaerobic bacterium Clostridium acetobutylicum has been considered a promising process of industrial biofuel production. Phosphotransbutyrylase (phosphate butyryltransferase, PTB) plays a crucial role in the butyrate metabolism by catalyzing the reversible conversion of butyryl-CoA into butyryl phosphate. Here, we report the crystal structure of PTB from the Clostridial host for ABE fermentation, C. acetobutylicum, (CaPTB) at a 2.9 A resolution. The overall structure of the CaPTB monomer is quite similar to those of other acyltransferase, with some regional structural differences. The monomeric structure of CaPTB consists of two distinct domains, the N- and C-terminal domains. The active site cleft was formed at the interface between two domains. Interestingly, the crystal structure of CaPTB contained eight molecules per asymmetric unit, forming an octamer, and the size-exclusion chromatography experiment also suggest that the enzyme exists as an octamer in solution. The structural analysis of CaPTB identifies the substrate binding mode of the enzyme and comparisons with other acyltransferase structures lead us to speculate that the enzyme undergoes a conformational change upon binding of its substrate. | ||
+ | |||
+ | Crystal structure and molecular mechanism of phosphotransbutyrylase from Clostridium acetobutylicum.,Kim S, Kim KJ J Microbiol Biotechnol. 2021 Sep 25;31(10):1-9. doi: 10.4014/jmb.2109.09036. PMID:34584034<ref>PMID:34584034</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7vg9" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Clostridium acetobutylicum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kim K-J]] | [[Category: Kim K-J]] | ||
[[Category: Kim S]] | [[Category: Kim S]] |
Current revision
Crystal structure of phosphotransbutyrylase from Clostridium acetobutylicum
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