1pey
From Proteopedia
(Difference between revisions)
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<StructureSection load='1pey' size='340' side='right'caption='[[1pey]], [[Resolution|resolution]] 2.25Å' scene=''> | <StructureSection load='1pey' size='340' side='right'caption='[[1pey]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1pey]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1pey]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PEY FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |
| - | <tr id=' | + | |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pey OCA], [https://pdbe.org/1pey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pey RCSB], [https://www.ebi.ac.uk/pdbsum/1pey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pey ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pey OCA], [https://pdbe.org/1pey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pey RCSB], [https://www.ebi.ac.uk/pdbsum/1pey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pey ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pey ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pey ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex. | ||
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| - | Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F.,Mukhopadhyay D, Sen U, Zapf J, Varughese KI Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):638-45. Epub 2004, Mar 23. PMID:15039551<ref>PMID:15039551</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1pey" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]] | *[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bacillus subtilis]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Mukhopadhyay | + | [[Category: Mukhopadhyay D]] |
| - | [[Category: Sen | + | [[Category: Sen U]] |
| - | [[Category: Varughese | + | [[Category: Varughese KI]] |
| - | [[Category: Zapf | + | [[Category: Zapf J]] |
| - | + | ||
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Current revision
Crystal structure of the Response Regulator Spo0F complexed with Mn2+
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