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| | <StructureSection load='2w1r' size='340' side='right'caption='[[2w1r]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='2w1r' size='340' side='right'caption='[[2w1r]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1R FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2w1t|2w1t]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1r OCA], [https://pdbe.org/2w1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1r RCSB], [https://www.ebi.ac.uk/pdbsum/2w1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1r ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1r OCA], [https://pdbe.org/2w1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1r RCSB], [https://www.ebi.ac.uk/pdbsum/2w1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SP5T_BACSU SP5T_BACSU]] Positive and negative transcriptional regulator of sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development.
| + | [https://www.uniprot.org/uniprot/SPOVT_BACSU SPOVT_BACSU] Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).<ref>PMID:22522895</ref> <ref>PMID:8755877</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Asen, I]] | + | [[Category: Asen I]] |
| - | [[Category: Djuranovic, S]] | + | [[Category: Djuranovic S]] |
| - | [[Category: Lupas, A N]] | + | [[Category: Lupas AN]] |
| - | [[Category: Zeth, K]] | + | [[Category: Zeth K]] |
| - | [[Category: Activator]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Regulatory gaf domain of spovt]]
| + | |
| - | [[Category: Repressor]]
| + | |
| - | [[Category: Sporulation]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Function
SPOVT_BACSU Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).[1] [2]
Publication Abstract from PubMed
Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the crystal structure of the isolated C-terminal domain of SpoVT at 1.5 A by experimental phasing techniques and used this model to solve the structure of the full-length SpoVT at 2.6 A by molecular replacement. SpoVT is a tetramer that shows an overall significant distortion mediated by electrostatic interactions. Two monomers dimerize via the highly charged N-terminal domains to form swapped-hairpin beta-barrels. These asymmetric dimers further tetramerize through the formation of mixed helix bundles between their C-terminal domains, which themselves fold as GAF (cGMP-specific and cGMP-stimulated phosphodiesterases, Anabaena adenylate cyclases, and Escherichia coli FhlA) domains. The combination of a swapped-hairpin beta-barrel with a GAF domain represents a novel domain architecture in transcription factors. The occurrence of SpoVT homologs throughout Bacilli and Clostridia demonstrates the ancestral origin of this factor in sporulation.
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis.,Asen I, Djuranovic S, Lupas AN, Zeth K J Mol Biol. 2008 Oct 30. PMID:18996130[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramirez-Peralta A, Stewart KA, Thomas SK, Setlow B, Chen Z, Li YQ, Setlow P. Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis. J Bacteriol. 2012 Jul;194(13):3417-25. PMID:22522895 doi:10.1128/JB.00504-12
- ↑ Bagyan I, Hobot J, Cutting S. A compartmentalized regulator of developmental gene expression in Bacillus subtilis. J Bacteriol. 1996 Aug;178(15):4500-7. PMID:8755877 doi:10.1128/jb.178.15.4500-4507.1996
- ↑ Asen I, Djuranovic S, Lupas AN, Zeth K. Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis. J Mol Biol. 2008 Oct 30. PMID:18996130 doi:S0022-2836(08)01363-6
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