1fmt

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{{STRUCTURE_1fmt| PDB=1fmt | SCENE= }}
{{STRUCTURE_1fmt| PDB=1fmt | SCENE= }}
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'''METHIONYL-TRNAFMET FORMYLTRANSFERASE FROM ESCHERICHIA COLI'''
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===METHIONYL-TRNAFMET FORMYLTRANSFERASE FROM ESCHERICHIA COLI===
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==Overview==
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Formylation of the methionyl moiety esterified to the 3' end of tRNA(f)Met is a key step in the targeting of initiator tRNA towards the translation start machinery in prokaryotes. Accordingly, the presence of methionyl-tRNA(f)Met formyltransferase (FMT), the enzyme responsible for this formylation, is necessary for the normal growth of Escherichia coli. The present work describes the structure of crystalline E.coli FMT at 2.0 A, resolution. The protein has an N-terminal domain containing a Rossmann fold. This domain closely resembles that of the glycinamide ribonucleotide formyltransferase (GARF), an enzyme which, like FMT, uses N-10 formyltetrahydrofolate as formyl donor. However, FMT can be distinguished from GARF by a flexible loop inserted within its Rossmann fold. In addition, FMT possesses a C-terminal domain with a beta-barrel reminiscent of an OB fold. This latter domain provides a positively charged side oriented towards the active site. Biochemical evidence is presented for the involvement of these two idiosyncratic regions (the flexible loop in the N-terminal domain, and the C-terminal domain) in the binding of the tRNA substrate.
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{{ABSTRACT_PUBMED_8887566}}
==About this Structure==
==About this Structure==
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[[Category: Initiator trna]]
[[Category: Initiator trna]]
[[Category: Translation initiation]]
[[Category: Translation initiation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:32:05 2008''

Revision as of 00:32, 1 July 2008

Image:1fmt.png

Template:STRUCTURE 1fmt

METHIONYL-TRNAFMET FORMYLTRANSFERASE FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 8887566

About this Structure

1FMT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of crystalline Escherichia coli methionyl-tRNA(f)Met formyltransferase: comparison with glycinamide ribonucleotide formyltransferase., Schmitt E, Blanquet S, Mechulam Y, EMBO J. 1996 Sep 2;15(17):4749-58. PMID:8887566

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