3b2m

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of the Major Pilin from Streptococcus pyogenes

Structural highlights

3b2m is a 3 chain structure with sequence from Streptococcus pyogenes serotype M1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.22Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PILIN_STRP1 Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many bacterial pathogens have long, slender pili through which they adhere to host cells. The crystal structure of the major pilin subunit from the Gram-positive human pathogen Streptococcus pyogenes at 2.2 angstroms resolution reveals an extended structure comprising two all-beta domains. The molecules associate in columns through the crystal, with each carboxyl terminus adjacent to a conserved lysine of the next molecule. This lysine forms the isopeptide bonds that link the subunits in native pili, validating the relevance of the crystal assembly. Each subunit contains two lysine-asparagine isopeptide bonds generated by an intramolecular reaction, and we find evidence for similar isopeptide bonds in other cell surface proteins of Gram-positive bacteria. The present structure explains the strength and stability of such Gram-positive pili and could facilitate vaccine development.

Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure.,Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN Science. 2007 Dec 7;318(5856):1625-8. PMID:18063798^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Manetti AG, Zingaretti C, Falugi F, Capo S, Bombaci M, Bagnoli F, Gambellini G, Bensi G, Mora M, Edwards AM, Musser JM, Graviss EA, Telford JL, Grandi G, Margarit I. Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation. Mol Microbiol. 2007 May;64(4):968-83. PMID:17501921 doi:http://dx.doi.org/MMI5704
↑ Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN. Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure. Science. 2007 Dec 7;318(5856):1625-8. PMID:18063798 doi:318/5856/1625

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3b2m"

@@ Line 3: / Line 3: @@
 <StructureSection load='3b2m' size='340' side='right'caption='[[3b2m]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3b2m]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Strp1 Strp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2M FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3b2m]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B2M FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spy0128 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301447 STRP1])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b2m OCA], [https://pdbe.org/3b2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b2m RCSB], [https://www.ebi.ac.uk/pdbsum/3b2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b2m ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PILIN_STRP1 PILIN_STRP1]] Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation.<ref>PMID:17501921</ref>
+[https://www.uniprot.org/uniprot/PILIN_STRP1 PILIN_STRP1] Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation.<ref>PMID:17501921</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/3b2m_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 28: / Line 28: @@
 </div>
 <div class="pdbe-citations 3b2m" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Pilin 3D structures|Pilin 3D structures]]
 == References ==
 <references/>
@@ Line 33: / Line 36: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Strp1]]
+[[Category: Streptococcus pyogenes serotype M1]]
-[[Category: Baker, E N]]
+[[Category: Baker EN]]
-[[Category: Coulibaly, F]]
+[[Category: Coulibaly F]]
-[[Category: Kang, H J]]
+[[Category: Kang HJ]]
-[[Category: Proft, T]]
+[[Category: Proft T]]
-[[Category: All-beta]]
-[[Category: Cell adhesion]]
-[[Category: Isopeptide]]
-[[Category: Pili]]
-[[Category: Structural protein]]

3b2m

From Proteopedia

Current revision

Crystal Structure of the Major Pilin from Streptococcus pyogenes

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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