1cqt
From Proteopedia
(New page: 200px<br /> <applet load="1cqt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cqt, resolution 3.20Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1cqt.gif|left|200px]]<br /> | + | [[Image:1cqt.gif|left|200px]]<br /><applet load="1cqt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1cqt" size=" | + | |
caption="1cqt, resolution 3.20Å" /> | caption="1cqt, resolution 3.20Å" /> | ||
'''CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT'''<br /> | '''CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the crystal structure, at 3.2 A, of a ternary complex | + | We have determined the crystal structure, at 3.2 A, of a ternary complex containing an OCA-B peptide, the Oct-1 POU domain, and an octamer DNA site. The OCA-B peptide binds in the major groove near the center of the octamer site, and its polypeptide backbone forms a pair of hydrogen bonds with the adenine base at position 5 of the octamer DNA. Numerous protein-protein contacts between the OCA-B peptide and the POU domain are also involved in the ternary complex. In particular, the hydrophobic surface from a short alpha-helix of OCA-B helps to stabilize the complex by binding to a hydrophobic pocket on the POU-specific domain. The structure of this ternary complex is consistent with previous biochemical studies and shows how peptide-DNA and peptide-protein contacts from OCA-B provide structural and functional specificity in the regulation of immunoglobulin transcription. |
==About this Structure== | ==About this Structure== | ||
| - | 1CQT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1CQT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cepek, K.]] | [[Category: Cepek, K.]] | ||
| - | [[Category: Chasman, D | + | [[Category: Chasman, D I.]] |
| - | [[Category: Pabo, C | + | [[Category: Pabo, C O.]] |
| - | [[Category: Sharp, P | + | [[Category: Sharp, P A.]] |
[[Category: gene regulation/dna]] | [[Category: gene regulation/dna]] | ||
[[Category: oca-b]] | [[Category: oca-b]] | ||
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[[Category: protein-dna interface]] | [[Category: protein-dna interface]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:46 2008'' |
Revision as of 10:08, 21 February 2008
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CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT
Overview
We have determined the crystal structure, at 3.2 A, of a ternary complex containing an OCA-B peptide, the Oct-1 POU domain, and an octamer DNA site. The OCA-B peptide binds in the major groove near the center of the octamer site, and its polypeptide backbone forms a pair of hydrogen bonds with the adenine base at position 5 of the octamer DNA. Numerous protein-protein contacts between the OCA-B peptide and the POU domain are also involved in the ternary complex. In particular, the hydrophobic surface from a short alpha-helix of OCA-B helps to stabilize the complex by binding to a hydrophobic pocket on the POU-specific domain. The structure of this ternary complex is consistent with previous biochemical studies and shows how peptide-DNA and peptide-protein contacts from OCA-B provide structural and functional specificity in the regulation of immunoglobulin transcription.
About this Structure
1CQT is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface., Chasman D, Cepek K, Sharp PA, Pabo CO, Genes Dev. 1999 Oct 15;13(20):2650-7. PMID:10541551
Page seeded by OCA on Thu Feb 21 12:08:46 2008
