7bb5
From Proteopedia
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==Crystal structure of anti-CRISPR protein AcrIF9== | ==Crystal structure of anti-CRISPR protein AcrIF9== | ||
| - | <StructureSection load='7bb5' size='340' side='right'caption='[[7bb5]]' scene=''> | + | <StructureSection load='7bb5' size='340' side='right'caption='[[7bb5]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CRISPR-Cas systems are prokaryotic adaptive immune systems that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes and phages gave rise to phage anti-CRISPR (Acr) proteins that act as a counter defence against CRISPR-Cas systems by inhibiting the effector complex. Here, we used a combination of bulk biochemical experiments, X-ray crystallography and single-molecule techniques to explore the inhibitory activity of AcrIF6 and AcrIF9 proteins against the type I-F CRISPR-Cas system from Aggregatibacter actinomycetemcomitans (Aa). We showed that AcrIF6 and AcrIF9 proteins hinder Aa-Cascade complex binding to target DNA. We solved a crystal structure of Aa1-AcrIF9 protein, which differ from other known AcrIF9 proteins by an additional structurally important loop presumably involved in the interaction with Cascade. We revealed that AcrIF9 association with Aa-Cascade promotes its binding to off-target DNA sites, which facilitates inhibition of CRISPR-Cas protection. | ||
| + | |||
| + | Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9.,Kupcinskaite E, Tutkus M, Kopustas A, Asmontas S, Jankunec M, Zaremba M, Tamulaitiene G, Sinkunas T Sci Rep. 2022 Sep 15;12(1):15548. doi: 10.1038/s41598-022-19797-y. PMID:36109551<ref>PMID:36109551</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bb5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Aggregatibacter actinomycetemcomitans serotype e str. SC1083]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kupcinskaite E]] | [[Category: Kupcinskaite E]] | ||
[[Category: Sinkunas T]] | [[Category: Sinkunas T]] | ||
[[Category: Tamulaitiene G]] | [[Category: Tamulaitiene G]] | ||
Revision as of 08:28, 7 December 2022
Crystal structure of anti-CRISPR protein AcrIF9
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