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2bkg

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Revision as of 19:28, 19 October 2022

Crystal structure of E3_19 a designed ankyrin repeat protein

Structural highlights

2bkg is a 2 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Consensus-designed ankyrin repeat (AR) proteins are thermodynamically very stable. The structural analysis of the designed AR protein E3_5 revealed that this stability is due to a regular fold with highly conserved structural motifs and H-bonding networks. However, the designed AR protein E3_19 exhibits a significantly lower stability than E3_5 (9.6 vs. 14.8 kcal/mol), despite 88% sequence identity. To investigate the structural correlations of this stability difference between E3_5 and E3_19, we determined the crystal structure of E3_19 at 1.9 A resolution. E3_19 as well has a regular AR domain fold with the characteristic H-bonding patterns. All structural features of the E3_5 and E3_19 molecules appear to be virtually identical (RMSD(Calpha) approximately 0.7 A). However, clear differences are observed in the surface charge distribution of the two AR proteins. E3_19 features clusters of charged residues and more exposed hydrophobic residues than E3_5. The atomic coordinates of E3_19 have been deposited in the Protein Data Bank. PDB ID: 2BKG.

Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability.,Binz HK, Kohl A, Pluckthun A, Grutter MG Proteins. 2006 Nov 1;65(2):280-4. PMID:16493627^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Binz HK, Kohl A, Pluckthun A, Grutter MG. Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability. Proteins. 2006 Nov 1;65(2):280-4. PMID:16493627 doi:http://dx.doi.org/10.1002/prot.20930

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bkg"

@@ Line 3: / Line 3: @@
 <StructureSection load='2bkg' size='340' side='right'caption='[[2bkg]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bkg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bkg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKG FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1k1a|1k1a]], [[1k1b|1k1b]], [[1mj0|1mj0]], [[1n0q|1n0q]], [[1n0r|1n0r]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkg OCA], [https://pdbe.org/2bkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bkg RCSB], [https://www.ebi.ac.uk/pdbsum/2bkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bkg ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkg OCA], [https://pdbe.org/2bkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bkg RCSB], [https://www.ebi.ac.uk/pdbsum/2bkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bkg ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 34: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Synthetic construct sequences]]
+[[Category: Synthetic construct]]
-[[Category: Binz, H K]]
+[[Category: Binz HK]]
-[[Category: Grutter, M G]]
+[[Category: Grutter MG]]
-[[Category: Kohl, A]]
+[[Category: Kohl A]]
-[[Category: Pluckthun, A]]
+[[Category: Pluckthun A]]
-[[Category: Ankyrin repeat]]
-[[Category: Consensus design]]
-[[Category: De novo protein]]
-[[Category: Designed protein]]
-[[Category: Protein stability]]

2bkg

From Proteopedia

Revision as of 19:28, 19 October 2022

Crystal structure of E3_19 a designed ankyrin repeat protein

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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