2jwt

From Proteopedia

(Difference between revisions)

Revision as of 08:38, 14 June 2023

Solution structure of Engrailed homeodomain WT

Structural highlights

2jwt is a 1 chain structure with sequence from Drosophila melanogaster. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HMEN_DROME This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Two methods are currently available to solve high resolution protein structures-X-ray crystallography and nuclear magnetic resonance (NMR). Both methods usually produce highly similar structures, but small differences between both solutions are always observed. Here the raw NMR data as well as the solved NMR structure were compared to the multiple crystal structures solved for the WT 60 residue three helix bundle engrailed homeodomain (EnHD) and single point mutants. There was excellent agreement between TALOS-predicted and crystal structure-observed dihedral angles and a good agreement for the (3) J(H ( N ) H ( alpha )) couplings for the multiple crystal structures. Around 1% of NOEs were violated for any crystal structure, but no NOE was inconsistent with all of the crystal structures. Violations usually occurred for surface residues or for residues for which multiple discreet conformations were observed between the crystal structures. Comparison of the disorder shown in the multiple crystal structures shows little correlation with dynamics under native conditions for this protein.

Comparison of multiple crystal structures with NMR data for engrailed homeodomain.,Religa TL J Biomol NMR. 2008 Mar;40(3):189-202. Epub 2008 Feb 15. PMID:18274703^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Religa TL. Comparison of multiple crystal structures with NMR data for engrailed homeodomain. J Biomol NMR. 2008 Mar;40(3):189-202. Epub 2008 Feb 15. PMID:18274703 doi:10.1007/s10858-008-9223-9

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jwt"

Categories: Drosophila melanogaster | Large Structures | Religa TL

@@ Line 1: / Line 1: @@
 ==Solution structure of Engrailed homeodomain WT==
-<StructureSection load='2jwt' size='340' side='right'caption='[[2jwt]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
+<StructureSection load='2jwt' size='340' side='right'caption='[[2jwt]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jwt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JWT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jwt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JWT FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1enh|1enh]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jwt OCA], [https://pdbe.org/2jwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jwt RCSB], [https://www.ebi.ac.uk/pdbsum/2jwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jwt ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">en ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jwt OCA], [https://pdbe.org/2jwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jwt RCSB], [https://www.ebi.ac.uk/pdbsum/2jwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jwt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME]] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
+[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Religa, T L]]
+[[Category: Religa TL]]
-[[Category: Developmental protein]]
-[[Category: Dna-binding]]
-[[Category: Homeobox]]
-[[Category: Homeodomain]]
-[[Category: Nucleus]]
-[[Category: Phosphorylation]]
-[[Category: Repressor]]
-[[Category: Segmentation polarity protein]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]

2jwt

From Proteopedia

Revision as of 08:38, 14 June 2023

Solution structure of Engrailed homeodomain WT

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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