1fsi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1fsi.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1fsi.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1fsi| PDB=1fsi | SCENE= }}
{{STRUCTURE_1fsi| PDB=1fsi | SCENE= }}
-
'''CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA'''
+
===CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA===
-
==Overview==
+
<!--
-
The crystal structure of the cyclic phosphodiesterase (CPDase) from Arabidopsis thaliana, an enzyme involved in the tRNA splicing pathway, was determined at 2.5 A resolution. CPDase hydrolyzes ADP-ribose 1",2"-cyclic phosphate (Appr&gt;p), a product of the tRNA splicing reaction, to the monoester ADP-ribose 1"-phosphate (Appr-1"p). The 181 amino acid protein shows a novel, bilobal arrangement of two alphabeta modules. Each lobe consists of two alpha-helices on the outer side of the molecule, framing a three- or four-stranded antiparallel beta-sheet in the core of the protein. The active site is formed at the interface of the two beta-sheets in a water-filled cavity involving residues from two H-X-T/S-X motifs. This previously noticed motif participates in coordination of a sulfate ion. A solvent-exposed surface loop (residues 100-115) is very likely to play a flap-like role, opening and closing the active site. Based on the crystal structure and on recent mutagenesis studies of a homologous CPDase from Saccharomyces cerevisiae, we propose an enzymatic mechanism that employs the nucleophilic attack of a water molecule activated by one of the active site histidines.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11080166}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11080166 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11080166}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Adp-ribose 1'',2''-cyclic phosphate]]
[[Category: Adp-ribose 1'',2''-cyclic phosphate]]
[[Category: Appr>p]]
[[Category: Appr>p]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:42:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:52:49 2008''

Revision as of 00:52, 1 July 2008

Image:1fsi.png

Template:STRUCTURE 1fsi

CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA

Template:ABSTRACT PUBMED 11080166

About this Structure

1FSI is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of activity of the cyclic phosphodiesterase of Appr>p, a product of the tRNA splicing reaction., Hofmann A, Zdanov A, Genschik P, Ruvinov S, Filipowicz W, Wlodawer A, EMBO J. 2000 Nov 15;19(22):6207-17. PMID:11080166[[Category: Adp-ribose 1,2-cyclic phosphate]] [[Category: Appr>p]]

Page seeded by OCA on Tue Jul 1 03:52:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fsi"
Personal tools