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2kmt

From Proteopedia

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Revision as of 08:45, 14 June 2023

NMR solution structure of Vibrio fischeri CcdB

Structural highlights

2kmt is a 2 chain structure with sequence from Aliivibrio fischeri. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q84B82_ALIFS

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show a dimer with a hydrophobic core crossing the dimer interface. In contrast to its F plasmid homologue, CcdB(Vfi) possesses a rigid dimer interface, and the apparent relative rotations of the two subunits are due to structural plasticity of the monomer. CcdB(Vfi) shows a number of non-conservative substitutions compared with the F plasmid protein in both the CcdA and the gyrase binding sites. Although variation in the CcdA interaction site likely determines toxin-antitoxin specificity, substitutions in the gyrase-interacting region may have more profound functional implications.

Structural and thermodynamic characterization of Vibrio fischeri CcdB.,De Jonge N, Hohlweg W, Garcia-Pino A, Respondek M, Buts L, Haesaerts S, Lah J, Zangger K, Loris R J Biol Chem. 2010 Feb 19;285(8):5606-13. Epub 2009 Dec 2. PMID:19959472^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ De Jonge N, Hohlweg W, Garcia-Pino A, Respondek M, Buts L, Haesaerts S, Lah J, Zangger K, Loris R. Structural and thermodynamic characterization of Vibrio fischeri CcdB. J Biol Chem. 2010 Feb 19;285(8):5606-13. Epub 2009 Dec 2. PMID:19959472 doi:10.1074/jbc.M109.068429

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kmt"

Categories: Aliivibrio fischeri | Large Structures | Zangger K

@@ Line 1: / Line 1: @@
 ==NMR solution structure of Vibrio fischeri CcdB==
-<StructureSection load='2kmt' size='340' side='right'caption='[[2kmt]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kmt' size='340' side='right'caption='[[2kmt]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kmt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_fischeri"_(beijerinck_1889)_bergey_et_al._1930 "achromobacter fischeri" (beijerinck 1889) bergey et al. 1930]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kmt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KMT FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccdB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668 "Achromobacter fischeri" (Beijerinck 1889) Bergey et al. 1930])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kmt OCA], [https://pdbe.org/2kmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kmt RCSB], [https://www.ebi.ac.uk/pdbsum/2kmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kmt ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kmt OCA], [https://pdbe.org/2kmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kmt RCSB], [https://www.ebi.ac.uk/pdbsum/2kmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kmt ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q84B82_ALIFS Q84B82_ALIFS]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 31: @@
 __TOC__
 </StructureSection>
+[[Category: Aliivibrio fischeri]]
 [[Category: Large Structures]]
-[[Category: Zangger, K]]
+[[Category: Zangger K]]
-[[Category: Toxin]]

2kmt

From Proteopedia

Revision as of 08:45, 14 June 2023

NMR solution structure of Vibrio fischeri CcdB

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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