1fvi

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[[Image:1fvi.gif|left|200px]]
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[[Image:1fvi.png|left|200px]]
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==Overview==
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{{ABSTRACT_11106756}}
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Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase known; it has an intrinsic nick-sensing function and suffices for yeast cell growth. Here, we report the 2.0 A crystal structure of the covalent ligase-AMP reaction intermediate. The conformation of the adenosine nucleoside and contacts between the enzyme and the ribose sugar have undergone a significant change compared to complexes of T7 ligase with ATP or mRNA capping enzyme with GTP. The conformational switch allows the 3' OH of AMP to coordinate directly the 5' PO(4) of the nick. The structure explains why nick sensing is restricted to adenylated ligase and why the 5' phosphate is required for DNA binding. We identify a metal binding site on ligase-adenylate and propose a mechanism of nick recognition and catalysis supported by mutational data.
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==About this Structure==
==About this Structure==
1FVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlorella_virus Chlorella virus]. The following page contains interesting information on the relation of 1FVI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVI OCA].
1FVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlorella_virus Chlorella virus]. The following page contains interesting information on the relation of 1FVI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVI OCA].
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==Reference==
 
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Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining., Odell M, Sriskanda V, Shuman S, Nikolov DB, Mol Cell. 2000 Nov;6(5):1183-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11106756 11106756]
 
[[Category: Chlorella virus]]
[[Category: Chlorella virus]]
[[Category: DNA Ligase]]
[[Category: DNA Ligase]]
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[[Category: Sriskanda, V.]]
[[Category: Sriskanda, V.]]
[[Category: Adenylated dna ligase]]
[[Category: Adenylated dna ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:48:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:23:41 2008''

Revision as of 14:23, 26 June 2008

Image:1fvi.png

Template:STRUCTURE 1fvi

CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE


Template:ABSTRACT 11106756

About this Structure

1FVI is a Single protein structure of sequence from Chlorella virus. The following page contains interesting information on the relation of 1FVI with [DNA Ligase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:23:41 2008

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