Sandbox Reserved 1693
From Proteopedia
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Amino acids His85, His105, Tyr150, Arg166, Ser170, Arg220, Pro223, Asn275, Arg331 are important in ligand to protein interaction. <scene name='89/892736/Key_amino_acids_with_rha/1'>Highlighted Amino Acids</scene>. <ref>PMID:34303708</ref> | Amino acids His85, His105, Tyr150, Arg166, Ser170, Arg220, Pro223, Asn275, Arg331 are important in ligand to protein interaction. <scene name='89/892736/Key_amino_acids_with_rha/1'>Highlighted Amino Acids</scene>. <ref>PMID:34303708</ref> | ||
- | His 85 | + | His 85, Arg166, Tyr 150 are threeamino acids that form hydrogen bonds with the Rha sugar. His 85 and Arg166 proved to be a key amino acid in the catalytic reaction that divides the Rha-GlcA complex in the binding pocket during the reaction. <scene name='89/892736/Highlighted_rha-glca/4'>Visual of Amino Acids here</scene>. His 105 showed to form a stabilizing hydrogen bond with His 85, reinforcing the catalytic importance of His 85. The Rha Sugar cap on the GA also interacts with amino acids Arg166, Ser170, Arg220 by Hbonding, which is an important part of separating Rha-GlcA. |
+ | |||
+ | Amino Acids Asn275 and Arg331 Hbond with the Glc, and they play an important role in stabilization during the catalytic mechanism. | ||
== Structural highlights == | == Structural highlights == | ||
Secondary Structural Highlights - | Secondary Structural Highlights - |
Revision as of 22:47, 8 December 2021
This Sandbox is Reserved from 10/01/2021 through 01/01//2022 for use in Biochemistry taught by Bonnie Hall at Grand View University, Des Moines, USA. This reservation includes Sandbox Reserved 1690 through Sandbox Reserved 1699. |
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==L-rhamnose-a-1,4-D-glucuronate lyase
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References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
- ↑ Kondo T, Kichijo M, Maruta A, Nakaya M, Takenaka S, Arakawa T, Fushinobu S, Sakamoto T. Structural and functional analysis of gum arabic l-rhamnose-alpha-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family. J Biol Chem. 2021 Jul 22:101001. doi: 10.1016/j.jbc.2021.101001. PMID:34303708 doi:http://dx.doi.org/10.1016/j.jbc.2021.101001
- ↑ Kondo T, Kichijo M, Maruta A, Nakaya M, Takenaka S, Arakawa T, Fushinobu S, Sakamoto T. Structural and functional analysis of gum arabic l-rhamnose-alpha-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family. J Biol Chem. 2021 Jul 22:101001. doi: 10.1016/j.jbc.2021.101001. PMID:34303708 doi:http://dx.doi.org/10.1016/j.jbc.2021.101001