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| - | [[Image:1fx0.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1fx0| PDB=1fx0 | SCENE= }} | | {{STRUCTURE_1fx0| PDB=1fx0 | SCENE= }} |
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| - | '''Crystal structure of the chloroplast F1-ATPase from spinach'''
| + | ===Crystal structure of the chloroplast F1-ATPase from spinach=== |
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| - | ==Overview==
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| - | The structure of the F(1)-ATPase from spinach chloroplasts was determined to 3.2 A resolution by molecular replacement based on the homologous structure of the bovine mitochondrial enzyme. The crystallized complex contains four different subunits in a stoichiometry of alpha(3)beta(3)gammaepsilon. Subunit delta was removed before crystallization to improve the diffraction of the crystals. The overall structure of the noncatalytic alpha-subunits and the catalytic beta-subunits is highly similar to those of the mitochondrial and thermophilic subunits. However, in the crystal structure of the chloroplast enzyme, all alpha- and beta-subunits adopt a closed conformation and appear to contain no bound adenine nucleotides. The superimposed crystallographic symmetry in the space group R32 impaired an exact tracing of the gamma- and epsilon-subunits in the complex. However, clear electron density was present at the core of the alpha(3)beta(3)-subcomplex, which probably represents the C-terminal domain of the gamma-subunit. The structure of the spinach chloroplast F(1) has a potential binding site for the phytotoxin, tentoxin, at the alphabeta-interface near betaAsp(83) and an insertion from betaGly(56)-Asn(60) in the N-terminal beta-barrel domain probably increases the thermal stability of the complex. The structure probably represents an inactive latent state of the ATPase, which is unique to chloroplast and cyanobacterial enzymes. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11032839}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11032839 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11032839}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Potential tentoxin binding site]] | | [[Category: Potential tentoxin binding site]] |
| | [[Category: Thermal stability]] | | [[Category: Thermal stability]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:51:39 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:04:22 2008'' |
Revision as of 01:04, 1 July 2008
Template:STRUCTURE 1fx0
Crystal structure of the chloroplast F1-ATPase from spinach
Template:ABSTRACT PUBMED 11032839
About this Structure
1FX0 is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
The structure of the chloroplast F1-ATPase at 3.2 A resolution., Groth G, Pohl E, J Biol Chem. 2001 Jan 12;276(2):1345-52. PMID:11032839
Page seeded by OCA on Tue Jul 1 04:04:22 2008
