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1fx5

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[[Image:1fx5.gif|left|200px]]
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{{STRUCTURE_1fx5| PDB=1fx5 | SCENE= }}
{{STRUCTURE_1fx5| PDB=1fx5 | SCENE= }}
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'''CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I'''
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===CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I===
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==Overview==
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The tertiary and quaternary structure of the lectin I from Ulex europaeus (UE-I) has been determined to 2.2 A resolution. UE-I is a dimeric metalloglycoprotein that binds the H-type 2 human blood group determinant [alpha-L-Fucalpha(1--&gt;2)-beta-D-Galbeta(1--&gt;4)-beta-D-Glc NAcalpha-]. Nine changes from the published amino acid sequence were necessary to account for the electron density. The quaternary structural organization of UE-I is that of the most commonly occurring legume lectin dimer. The tertiary structure of the monomeric subunits is similar to that in the conventional lectin subunit; however, some structural differences are noted. These differences include a four-stranded anti-parallel "S" sheet in UE-I versus the five-stranded S sheet in other lectin monomers. The Ala residue of the Ala-Asp cis-peptide bond present in the carbohydrate-binding site of the conventional lectin monomer is replaced with a Thr in the UE-I structure. Also, a novel disulfide bridge linking Cys115 and Cys150 is present. There are two metallic ions, one calcium and the other manganese, per subunit. N-linked oligosaccharides are at residues 23 and 111 of each subunit. One molecule of R-2-methyl-2, 4-pentanediol (R-MPD) is present in a shallow depression on the surface of each subunit. In order to examine the binding of the H-type 2 blood group determinant by UE-I, its beta-methyl glycoside (H-type 2-OMe) was docked into the binding site of R-MPD. The epitope previously identified for H-type 2-OMe by chemical mapping proved, with only minor adjustment of amino acid residues, to be complementary to the shallow cavity occupied by R-MPD in the structure. Several key interactions have been proposed between the H-type 2-OMe and UE-I.
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(as it appears on PubMed at http://www.pubmed.gov), where 11090284 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11090284}}
==About this Structure==
==About this Structure==
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[[Category: Legume lectin]]
[[Category: Legume lectin]]
[[Category: Ue-i]]
[[Category: Ue-i]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:52:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:04:43 2008''

Revision as of 01:04, 1 July 2008

Image:1fx5.png

Template:STRUCTURE 1fx5

CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I

Template:ABSTRACT PUBMED 11090284

About this Structure

1FX5 is a Single protein structure of sequence from Ulex europaeus. Full crystallographic information is available from OCA.

Reference

The 2.2 A resolution structure of the O(H) blood-group-specific lectin I from Ulex europaeus., Audette GF, Vandonselaar M, Delbaere LT, J Mol Biol. 2000 Dec 1;304(3):423-33. PMID:11090284

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