7e3g
From Proteopedia
(Difference between revisions)
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==Crystal structure of Trypanosoma brucei cathepsin B R91C/T223C mutant in the living cell== | ==Crystal structure of Trypanosoma brucei cathepsin B R91C/T223C mutant in the living cell== | ||
| - | <StructureSection load='7e3g' size='340' side='right'caption='[[7e3g]]' scene=''> | + | <StructureSection load='7e3g' size='340' side='right'caption='[[7e3g]], [[Resolution|resolution]] 3.86Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e3g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3g OCA], [https://pdbe.org/7e3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3g RCSB], [https://www.ebi.ac.uk/pdbsum/7e3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3g ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.86Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3g OCA], [https://pdbe.org/7e3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3g RCSB], [https://www.ebi.ac.uk/pdbsum/7e3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D6XHE1_TRYB2 D6XHE1_TRYB2] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells. | ||
| + | |||
| + | Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.,Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T Angew Chem Int Ed Engl. 2021 May 25;60(22):12341-12345. doi:, 10.1002/anie.202102039. Epub 2021 Apr 23. PMID:33759310<ref>PMID:33759310</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e3g" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cathepsin 3D structures|Cathepsin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma brucei brucei TREU927]] | ||
[[Category: Abe S]] | [[Category: Abe S]] | ||
[[Category: Hirata K]] | [[Category: Hirata K]] | ||
Current revision
Crystal structure of Trypanosoma brucei cathepsin B R91C/T223C mutant in the living cell
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